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Rat brain normal tissue lysate - membrane extract suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

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Western blot - Rat brain normal tissue lysate - membrane extract (AB29473), expandable thumbnail

Key facts

Form
Liquid

Target data

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Rat brain normal tissue lysate - membrane extract suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

Key facts

Form
Liquid
Concentration
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Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Allow to warm to room temp and agitate gently before aliquoting

Notes


Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC.

Quality control:
1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot.
2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Rat brain normal tissue lysate - membrane extract (ab29473), expandable thumbnail

    Western blot - Rat brain normal tissue lysate - membrane extract (ab29473)

    All lanes: Western blot - Anti-SHANK3 antibody [N69/46] (Anti-SHANK3 antibody [N69/46] ab193307) at 5 µg/mL

    Lane 1: Cerebellum Mouse Tissue Lysate at 20 µg

    Lane 2: Rat Cortex at 20 µg

    Lane 3: Western blot - Rat brain normal tissue lysate - membrane extract (ab29473) at 20 µg

    Lane 4: Human Cortex at 20 µg

    Lane 5: Human Striatum Neuronal cell lysate at 20 µg

    Lane 6: Human liver tissue lysate - total protein (ab29889) at 20 µg

    Secondary

    All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 186 kDa

    Observed band size: 186 kDa

    Exposure time: 4min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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