Rat heart tissue lysate - mitochondrial extract suitable for WB, SDS-PAGE, IP. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
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Rat heart tissue lysate - mitochondrial extract suitable for WB, SDS-PAGE, IP. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
Product was previously marketed under the MitoSciences sub-brand.
2 mg of rat heart mitochondria in 400ul of iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE. Immunocapture, however could also be used for electrophoresis and Western blotting, and Enzyme Isolations. 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 μl of the mitochondrial membranes supplied be solubilized by the addition of 20 μl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.
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