Rat liver tissue lysate - mitochondrial extract

Product was previously marketed under the MitoSciences sub-brand.

2 mg of purified rat liver mitochondrial membranes resuspended in 400 μl of heart mitochondria resuspension buffer (10 mM Tris.HCl, pH 7.8, 0.2 M sucrose, 0.2 mM EDTA, 1 mM PMSF). 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 μl of the mitochondrial membranes supplied be solubilized by the addition of 20 μl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.