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THP-1 nuclear extract lysate (PMA stimulated). View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

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Functional Studies - THP-1 nuclear extract lysate (PMA stimulated) (AB14901), expandable thumbnail

Key facts

Cell type
THP-1
Species or organism
Human
Tissue
Blood
Form
Liquid

Target data

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THP-1 nuclear extract lysate (PMA stimulated). View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

Key facts

Cell type
THP-1
Form
Liquid
Disease
Acute Monocytic Leukemia
Concentration
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Cell culture

Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Male

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Cell line: THP1 (Human acute monocytic leukemia).
The protein content has been determined by a Bradford-based assay.
The THP-1 nuclear extracts have been collected in Lysis Buffer after a 24-hour incubation with or without PMA (10 ng/ml).

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1 product image

  • Functional Studies - THP-1 nuclear extract lysate (PMA stimulated) (ab14901), expandable thumbnail

    Functional Studies - THP-1 nuclear extract lysate (PMA stimulated) (ab14901)

    Each lot has been tested for PPAR-gamma activation by using TransAM™ PPAR-gamma Kits. The signal intensity for PPAR-gamma activation in each lot is compared to the signal intensity obtained with extracts from unstimulated THP-1 cells (see figure). After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of PPAR-gamma activation in a given cell type.

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Product protocols

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