THP-1 nuclear extract lysate (PMA stimulated)
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Functional Studies - THP-1 nuclear extract lysate (PMA stimulated) (AB14901)
Each lot has been tested for PPAR-gamma activation by using TransAM™ PPAR-gamma Kits. The signal intensity for PPAR-gamma activation in each lot is compared to the signal intensity obtained with extracts from unstimulated THP-1 cells (see figure). After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of PPAR-gamma activation in a given cell type.
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Product details
The protein content has been determined by a Bradford-based assay.
The THP-1 nuclear extracts have been collected in Lysis Buffer after a 24-hour incubation with or without PMA (10 ng/ml).
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Male
Product promise
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