WI-38 nuclear extract lysate (forskolin stimulated). View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
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Key facts
- Cell type
- WI-38
- Species or organism
- Human
- Form
- Liquid
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WI-38 nuclear extract lysate (forskolin stimulated). View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
Key facts
- Cell type
- WI-38
- Species or organism
- Human
- Form
- Liquid
- Concentration
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
The WI-38 nuclear extracts have been collected after a 30-minute incubation with or without forskolin (20 μM). The protein content has been determined by a Bradford-based assay.
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Functional Studies - WI-38 nuclear extract lysate (forskolin stimulated) (ab14912)
Each lot has been tested for CREB activation by using TransAM™ pCREB Kits. The signal intensity for CREB activation in each lot is compared to the signal intensity obtained with extracts from unstimulated WI-38 VA13 cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 3. This ratio may vary depending on the basal level of CREB activation in a given cell type.
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