This data was developed using ab283913, the same antibody clone in a different buffer formulation.

Lane 1: Red – loading control Donkey anti-Goat IgG H&L (IRDye^® 800CW) preadsorbed (ab216775) binding to the sheep Fc Chimera tag on recombinant human coronavirus SARS-CoV-2 spike glycoprotein S2 – observed at 135 kDa

Lanes 2: Green – ab283913 observed at 135 kDa.

ab283913 was shown to bind specifically to SARS-CoV-2 spike glycoprotein S2 in Western blot. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Donkey anti-Goat IgG H&L (IRDye^® 800CW) preadsorbed (ab216775) and Donkey anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216779) at 1/20000 dilution.

Blocking buffer: 3% milk in TBS-0.1% Tween^® 20 (TBS-T)

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