Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and no reporter gene to allow construction of user's own system.
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- Plant science : an international journal of experimental plant biology 306:1108742021PpEBB1 directly binds to the GCC box-like element of auxin biosynthesis related genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuehui Zhao,Binbin Wen,Chen Li,Li Liu,Xiude Chen,Dongmei Li,Ling Li,Xiling FuPubMed 33775370
- eLife 4:2015Structural basis of pathogen recognition by an integrated HMA domain in a plant NLR immune receptor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA Maqbool et. al.PubMed 26304198
Key facts
- Target
- pCambia1300
- Form
- Lyophilized
Target data
Recommended products
Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and no reporter gene to allow construction of user's own system.
Key facts
- Target
- pCambia1300
- Form
- Lyophilized
- Storage buffer
Constituents: 71% Tris, 17% EDTA
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and no reporter gene to allow construction of user's own system.
The pCambia1300 vector offers:
High copy number in E.coli for high DNA yields
pVS1 replicon for high stability in Agrobacterium
Small size
Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with kanamycin
Plant selection with hygromycin B
Plant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV35S promoter and terminated by the CaMV35S polyA signal.
This vector contains minimal heterologous sequences for plant transformation and selection of transformants, allowing insertion of desired genes for transformation into plants but require all promoter and terminator sequences for plant expression of newly cloned genes.
The pUC18 polylinker within the lacZa fragment allows blue/white screening of clones in E. coli cloning work.
Concentration of Antibiotics for selection.
In bacterial system:
Kanamycin resistance (aphIII/nptIII/3'5''-aminoglycoside phosphotransferase typeIII from
Enterococcus faecalis pJH1) in bacteria: use 50 μg/mL in E.coli and A.tumefaciens.
In plant system:
| Resistance Gene | hph (From a Klebsiella species, via E.coli) |
| Transformed plant selection antibiotic | Hygromycin B |
| Concentration in Arabidopsis | 50 μg/mL in germination medium |
| Concentration in Rice | 50 μg/mL in 2N6 then in RGH6 |
| Concentration in Tobacco | 50 μg/mL in RMOP then in MST |
Product promise
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Vector - pCambia1300 Plant Expression Vector (ab275754)
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