We know that if you’re working in a high-throughput setting, you need reproducible, sensitive assays that allow you to measure a high number of samples. Transitioning from low to high throughput presents myriad challenges: assays must retain the same excellent accuracy, precision, linearity, and detection limits, giving crucial reproducible results.

Developing assays is time-consuming and labor-intensive. Spending valuable time and effort developing what looks like the perfect assay, only to find out it fails reproducibility benchmarks, represents a colossal risk.

To help you mitigate this risk and scale up your assays with confidence, you need extremely robust, high-throughput, automation-friendly assays. That’s why we developed a 384-well format for our SimpleStep ELISA® kits, purpose-built to allow you to succeed when rapidly processing large numbers of samples.

Same principle

The 384-well SimpleStep ELISA® uses a different plate format to our standard 96-well kits, but the principle is the same. The assay procedure uses exactly the same simple mix, wash, and read steps, powered by the same CaptSure™ technology. This means our 384-well format has the same 90-minute assay time, saving you crucial time at the bench. The majority of our kits use rabbit monoclonal antibody pairs, purpose-built for ELISA. Recombinant technology means our kits can be manufactured ab infinitum, giving you guaranteed supply and batch-to-batch consistency, so you can trust that your kit will give you the same data every time you order.

Same results

Our 384-well kits are powered by the same antibody pairs and protein standards as our 96-well kits, guaranteeing the same excellent sensitivity, specificity, and reproducibility you’ve come to expect.

“One of the things I’m most proud of is the compatibility with our 96-well kits. We use the same antibodies and diluents as our 96-well kits, so customers using the 96-well format can easily convert over to 384-well. Customers can develop and optimize their methods in a manual-friendly way, then convert over to the 384-well format to really crank through their sample set.”

Dr Jeff Monette, Head of Immunoassay Development at Abcam

We ensure that our 384-well kits are validated against our 96-well kits, ensuring both our usual rigorous quality standards and inter-assay reproducibility. We have strict standard curve and biological reproducibility criteria to ensure that your transition from the 96- to 384-well format is truly seamless. Biological interpolated values are within 10% between the 384- and 96-well kits, meaning that for a given sample you’ll measure the same target concentration regardless of plate format. Full validation guarantees low inter-assay variability, so you can be confident when comparing your results between labs.

“We were able to upvalidate many of the key target kits that our high-throughput customers need. There are already over 200 384-well SimpleStep ELISA® kits available with validation data showing compatibility with the 96-well kit.”

Dr Jeff Monette

Higher throughput

Sample volume for the 384-well format is just 12.5 μL, compared to 50 μL for the 96-well plate, maximizing your data collection and helping you preserve precious samples. You can get more done with less, collecting four times as much data with only a quarter of the sample volume.

The 384-well SimpleStep ELISA® is a ready-made, high-throughput engine purpose-built to drive you to your results faster. Discover our range of kits and find the best tool for your high-throughput workflow.