At Abcam, our mission is to save you time with high-quality tools that move your research forward, taking work off your hands so you can focus on delivering results.

To empower immunoassay designers, we’re constantly developing new recombinant rabbit monoclonal matched antibody pairs, each selected for their targets from multiple clone combinations and validated in biological samples to ensure the best specificity.

We now have 1,700+ carrier-free antibody pairs in an ever-growing range, also available as individual capture and detector antibodies, so you can find exactly what you need.

Thanks to our many partnerships with platform developers, we also have extensive data and expertise on how to optimize your multiplex panels using these antibodies. We can share that know-how with you to avoid cross-reactivity and interference and get the best multiplex results.

How we make a recombinant monoclonal matched antibody pair:

1. Hundreds of multiple candidates are screened for optimal performance.
2. Promising pairs are then tested for sensitivity, optimal orientation, and detection in biological samples.
3. The best two pairs are tested in both orientations with the full standard curve and all required biologicals. Linearity is calculated and the sample reading value is confirmed.
4. Cross-reactivity testing against alternative species and related proteins.
5. The optimal pair is confirmed based on sensitivity, linearity, and cross-reactivity.

“Choosing a high-performing antibody pair is at the heart of ELISA development. Specificity and batch-to-batch consistency are key to success or failure.”

– Andrew Ball, Vice President of Assay Development and Conjugates R&D, Abcam

We use a dedicated development pipeline to ensure all our antibody pairs are optimized for sandwich immunoassays, with a focus on quality that delivers major advantages:

Recombinant technology brings unrivaled specificity, sensitivity and reproducibility

All of our antibody pairs are recombinant monoclonal antibodies, made by cloning antibody genes into high-yield expression vectors and introducing them into hosts. Our recombinant antibodies deliver key advantages, including batch-to-batch consistency, confirmed specificity, and ease of scalability.

By using a unique set of genes, recombinant antibody production stays consistent and reliable long-term and avoids genetic drift.

Recombinants can also succeed where polyclonal and traditional monoclonal antibodies fall short, making challenging targets such as toxins, nucleotides, and membrane-bound proteins possible.

Biophysical characterization guarantees long-term batch-to-batch consistency

We use biophysical quality control (QC) to confirm antibody identity at a molecular level. We use this data to validate future batches, assessing their purity, aggregation, and sequence identity and integrity using liquid chromatography-mass spectrometry (LC-MS) and high-performance liquid chromatography (HPLC).

This provides a unique ‘fingerprint’ for eliminating lot-to-lot variability, meaning your assays can be reproduced again and again, any time, in any lab. To date, 75% of our recombinant antibodies have undergone biophysical QC – winning Abcam a CiteAb award* in 2024.

Our antibody pairs are conjugation-ready, so you can use them in any assay or platform

As well as 1,700+ carrier-free antibody pairs, we offer 3,500+ individual capture and detector antibodies, so you can take the concentration you want without wasting money on unneeded kits and pairs. As they’re BSA and azide-free, you can adapt them to whatever assay or platform you need, using our conjugation kits, if you like. Our antibody pairs can be used on many commonly used formats:

• Sandwich ELISA
• FRET/TR-FRET
• Bead-based assays
• AlphaLISA®/AlphaScreen®
• DELFIA® immunoassays
• Meso Scale Discovery® (MSD®)
• Simoa®
• Single Molecule Counting (SMC™) immunoassays

Our ELISA kits use the same antibodies, for flexibility and freedom to scale

Our matched antibody pairs are the same pairs used in our 96-well and 384-well SimpleStep ELISA® kits, with the same specificity, making them ideal for tailoring your ELISA assays to your own plate systems, and scaling throughput up or down. We also offer a growing range of complementary recombinant protein standards.

“We’re very happy to partner with Abcam to access their extensive portfolio of recombinant monoclonal antibody pairs, which accelerate our ability to bring our proteomic discovery tools to researchers."

– Dr Yuling Luo, CEO, Alamar Biosciences

Trusted by platform developers, our multiplex know-how minimizes cross-reactivity

We’re proud of our extensive partnerships with platform developers, including Akoya Biosciences, Alamar Biosciences, NanoString, Revvity, and many others. Thanks to these and our own in-house development, we’ve validated our antibody pairs on hundreds of panels across a wide range of multiplex platforms.

These include key targets in immuno-oncology, oncology, neurodegenerative diseases, infectious diseases, immunology, and more. You can benefit from our data, experience and expertise to optimize your panels, minimize cross-reactivity and interference, and move forward swiftly.

Antibodies that keep you moving forward

Our matched antibody pairs provide unmatched specificity, sensitivity, and reproducibility, empowering you with reliable and scalable solutions. Explore the possibilities with our extensive range and advance your immunoassay development with confidence.

*Highly Commended Digital Science Tool of the Year award, CiteAb, 2024.

Meso Scale Discovery® (MSD®) is a registered trademark of Meso Scale Diagnostic, LLC.
AlphaLISA®/AlphaScreen® and DELFIA® are registered trademarks of Perkin Elmer, Inc.
Simoa® is a registered trademark of Quanterix.
SMC™ is a trademark of Merck, KGaA, Darmstadt, Germany.

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