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Experiment support

Resources to get your experiments up and running.

Tips and technical resources to kickstart your experiments and keep them moving forward, including concentration calculations, using centrifuges, sample management, and more.

How do I determine protein concentration?

The quickest way to estimate the amount of protein in solution is to use UV-vis to measure absorbance directly, but this is generally not very accurate or sensitive. Highly accurate quantitation of most proteins can be achieved using either a Bradford or bicinchoninic acid (BCA) assay.

How should I measure samples from a microplate?

Many kits will detect samples using a standard microplate format. Your plate choice will depend upon the detection method. Please see below for a summary of the plate types we recommend for each type of reading:

How do I convert rpm to g when using a centrifuge?

Revolutions per minute (rpm) and g-force (g) are both used to describe the force exerted by a centrifuge when you’re spinning a sample. In the scientific literature, these units are often used interchangeably. If you want to replicate somebody’s experiment, it’s useful to understand how to convert one to the other.

Will this antibody cross-react?

When selecting an antibody, it's important to ensure it is specific to the target and does not cross-react. Find out what you need to know to minimize cross-reactivity.

How do I convert between different concentrations?

Converting between concentrations can be a headache. To make this easier, see the table below for some common units you might encounter when using one of our kits. You need to know the substance’s molecular weight (Mw) to convert between units based on mass and moles.

How do I calculate the dynamic range of an ELISA?

The dynamic range of an ELISA is the range of antigen concentrations that can be measured accurately by the assay.​ Read on for more on how dynamic range is determined and how to interpret it for different sample types.

How do I calculate values from a standard curve?

A standard curve is used to accurately determine the concentration of your sample from the signal generated by an assay. The signal is never perfectly proportional to the sample concentration. A standard curve is designed to correct for these effects, so you know which concentration a given signal value corresponds to.

How do I cite Abcam products in my published research?

Information on how to cite our products and leave reviews.

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