ACSL4
GeneName
ACSL4
Summary
ACSL4, also known as ACS4, FACL4, or long chain acyl CoA synthetase 4, is a 79 kDa enzyme predominantly expressed in the cytoplasm and associated with various cellular membranes, including the endoplasmic reticulum, mitochondria, and peroxisomes. It plays a crucial role in fatty acid metabolism by catalysing the conversion of long-chain fatty acids into their corresponding acyl-CoA derivatives. This enzymatic activity is essential for lipid biosynthesis and metabolism, particularly in processes involving arachidonic acid and other unsaturated fatty acids. ACSL4 is also implicated in cell growth regulation and insulin secretion, highlighting its involvement in metabolic pathways critical for cellular function.
Importance
ACSL4 is relevant to: - Lipid metabolism and the biosynthesis of long-chain fatty acids, which are vital for membrane integrity and energy storage - Regulation of prostaglandin secretion, influencing inflammatory responses and cellular signalling - Neuron differentiation, suggesting a role in neurodevelopment and potential implications in neurodegenerative diseases - The metabolic processes associated with pregnancy, indicating its importance in reproductive biology - Insulin secretion regulation, linking it to metabolic disorders such as diabetes
Top Products
For researchers investigating ACSL4, we highly recommend the top-selling recombinant antibody, Anti-FACL4 antibody [EPR8640] (ab155282). This antibody has been validated for a variety of applications, including Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC), making it a versatile tool for your research needs. With 345 citations, it is well-regarded in the scientific community, reflecting its reliability and effectiveness in detecting ACSL4. This recombinant antibody ensures batch-to-batch consistency, providing confidence in your experimental results.
Abcam Product Citation Summary
The data indicates a strong focus on the role of ACSL4 in human liver cancer, particularly hepatocellular carcinoma (HCC). Multiple studies utilise the same antibody, ab155282, for both western blotting and immunohistochemistry, highlighting its relevance in understanding cancer outcomes, progression, and the stability of c-Myc. Additionally, another antibody, ab205199, is employed in the context of ferroptosis in cancer cell lines, suggesting a broader interest in the metabolic pathways associated with ACSL4 in cancer research.
Abcam Product Citation Table
Function
Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed:21242590, PubMed:22633490, PubMed:24269233). Preferentially activates arachidonate and eicosapentaenoate as substrates (PubMed:21242590). Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs (By similarity). Modulates prostaglandin E2 secretion (PubMed:21242590).
Involvement in disease
Intellectual developmental disorder, X-linked 63
XLID63
A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. Intellectual deficiency is the only primary symptom of non-syndromic X-linked intellectual disability, while syndromic forms presents with associated physical, neurological and/or psychiatric manifestations.
None
The disease is caused by variants affecting the gene represented in this entry.
AMME complex
ATS-MR
An X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, sensorineural hearing loss, intellectual disability, midface hypoplasia and elliptocytosis.
None
The gene represented in this entry may be involved in disease pathogenesis.
Sequence Similarities
Belongs to the ATP-dependent AMP-binding enzyme family.
Cellular localization
- Mitochondrion outer membrane
- Single-pass type III membrane protein
- Peroxisome membrane
- Single-pass type III membrane protein
- Microsome membrane
- Single-pass type III membrane protein
- Endoplasmic reticulum membrane
- Single-pass type III membrane protein
- Cell membrane
Alternative names
ACS4, FACL4, LACS4, ACSL4, Long-chain-fatty-acid--CoA ligase 4, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 4, LACS 4