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ANPEP

GeneName

ANPEP

Summary

ANPEP, also known as gp130, APN, or CD13, is a 110 kDa type II membrane protein that is expressed in various tissues, including the kidney, lung, and heart. It is localised to the plasma membrane and other cellular compartments such as the cytoplasm and endoplasmic reticulum-Golgi intermediate compartment. ANPEP functions primarily as an alanyl aminopeptidase, involved in the cleavage of amino acids from the N-terminus of peptides. It also plays a role in peptide binding and is implicated in several biological processes, including angiogenesis, cell differentiation, and proteolysis. Additionally, ANPEP acts as a receptor for certain viruses, highlighting its importance in host-pathogen interactions.

Importance

ANPEP is relevant to: - Angiogenesis and tissue remodelling, impacting wound healing and tumour growth - Regulation of blood pressure through its role in angiotensin maturation - Immune responses, as it influences cell differentiation and peptide catabolism - Viral pathogenesis, given its function as a receptor for specific viruses

Top Products

For researchers investigating ANPEP, we highly recommend the top-selling recombinant antibody, Anti-CD13 antibody [EPR4058] (ab108310). This antibody has been validated in knockout models, ensuring its reliability in various applications, including immunohistochemistry (IHC), western blotting (WB), and immunocytochemistry (ICC). With 36 citations, it is well-regarded in the research community, making it an excellent choice for those seeking dependable ANPEP detection. The Human CD13 ELISA Kit (ab239427), supported by 1 citation, is an excellent option for researchers looking to measure ANPEP levels in their samples.

Abcam Product Citation Summary

The data indicates that ANPEP is being studied in the context of liver cancer stem cells and cell differentiation, particularly in human and mouse models. The use of various applications such as Western blot and immunofluorescence highlights the importance of ANPEP in understanding resistance mechanisms in cancer and the differentiation processes of stem cells.

Abcam Product Citation Table

Product Code
Species
Application
Study Context
PMID
ab154116
Mouse
WB
Liver cancer stem cells
31171015
ab154116
Human
WB
Liver cancer stem cells
31171015
ab7417
Human
WB, IF
Potential BRAFi resistance
29048432
ab7417
Human
WB, ICC-IF
Cell differentiation
29096715
ab7417
Human
WB, ICC-IF
WJ-MSC differentiation into EEC-like cells
29096715

Function

Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity (By similarity).

(Microbial infection) Acts as a receptor for human coronavirus 229E/HCoV-229E. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein.

(Microbial infection) Mediates as well Human cytomegalovirus (HCMV) infection.

Post-translational modifications

Sulfated.

N- and O-glycosylated.

May undergo proteolysis and give rise to a soluble form.

Sequence Similarities

Belongs to the peptidase M1 family.

Tissue Specificity

Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.

Cellular localization

Alternative names

CD13, APN, PEPN, ANPEP, Aminopeptidase N, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, AP-M

swissprot:P15144 entrezGene:290 entrezGene:929 swissprot:P08571 omim:158120 omim:151530