ANXA2
GeneName
ANXA2
Summary
ANXA2, also known as annexin II or p36, is a 39 kDa calcium-dependent phospholipid-binding protein that is widely expressed in various tissues, including the cytoplasm and plasma membrane. It plays a significant role in cell-cell adhesion through its interaction with cadherins and is involved in multiple cellular processes such as angiogenesis, exocytosis, and membrane repair. ANXA2 is localised to several cellular components, including adherens junctions, endosomes, and the extracellular matrix, where it participates in collagen fibril organisation and cell-matrix adhesion. Additionally, it can form complexes with other proteins, such as the AnxA2-p11 complex, and is implicated in regulating receptor-mediated endocytosis and cholesterol transport.
Importance
ANXA2 is relevant to: - Angiogenesis and tissue repair, influencing vascular development and regeneration - Lipid metabolism and atherogenesis through its role in low-density lipoprotein particle clearance - Neurogenesis and neuronal health, potentially affecting neurodevelopmental processes - Cancer biology, where it may contribute to tumour progression and metastasis due to its involvement in cell adhesion and migration - Immune responses, as it regulates exocytosis and vesicle fusion in immune cells.
Top Products
For researchers investigating ANXA2, we recommend two excellent primary antibodies. The first is the well-cited polyclonal antibody, Anti-Annexin-2/ANXA2 antibody (ab41803), which has garnered 80 citations, reflecting its reliability in the field. This antibody is particularly effective for Western blotting (WB), immunohistochemistry (IHC), and immunocytochemistry (ICC). Additionally, we offer the recombinant antibody, Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677), which is validated for a broader range of applications, including WB, IHC, ICC, and flow cytometry (FC). With 36 citations, this recombinant product provides the batch-to-batch consistency that many researchers seek. Together, these antibodies provide robust options for studying ANXA2 in various experimental contexts. The Anti-Annexin-2/ANXA2 ELISA Kit (ab178677), supported by 36 citations, is an excellent option for researchers looking to accurately measure ANXA2 levels in their samples.
Abcam Product Citation Summary
The data indicates that ANXA2 is being studied in various contexts, including lymphocyte egress, pulmonary artery tissue in CTEPH patients, and viral infections. The use of different applications such as immunofluorescence, immunohistochemistry, and Western blotting highlights the versatility of ANXA2 as a target in both mouse and human studies.
Abcam Product Citation Table
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid.
Function
Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9 (PubMed:18799458, PubMed:22848640, PubMed:24808179). Binds to endosomes damaged by phagocytosis of particulate wear debris and participates in endosomal membrane stabilization, thereby limiting NLRP3 inflammasome activation (By similarity). Required for endothelial cell surface plasmin generation and may support fibrinolytic surveillance and neoangiogenesis (By similarity).
(Microbial infection) Binds M.pneumoniae CARDS toxin, probably serves as one receptor for this pathogen. When ANXA2 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen.
Involvement in disease
Increased expression of ANXA2 is associated with hepatocellular carcinoma tumor progression.
Post-translational modifications
Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
ISGylated.
Sequence Similarities
Belongs to the annexin family.
Cellular localization
- Secreted
- Extracellular space
- Extracellular matrix
- Basement membrane
- Melanosome
- In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
Alternative names
ANX2, ANX2L4, CAL1H, LPC2D, ANXA2, Annexin A2, Annexin II, Annexin-2, Calpactin I heavy chain, Calpactin-1 heavy chain, Chromobindin-8, Lipocortin II, Placental anticoagulant protein IV, Protein I, p36, PAP-IV