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APAF1

OverviewProducts

Domain

The CARD domain mediates interaction with APIP.

The monomeric form is autoinhibited in a closed conformation through a bound ADP at the nucleotide binding site. Exchange of ADP for ATP and binding of cytochrome c trigger a large conformational change where the first WD repeat region swings out, allowing the NB-ARC domain to rotate and expose the contact areas for oligomerization (By similarity).

Function

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

Tissue specificity

Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.

Cellular localization

  • Cytoplasm

Alternative names

  • Apoptotic protease-activating factor 1
  • APAF-1
  • KIAA0413
  • APAF1

Database links

Target type

Proteins

Primary research area

Oncology

Molecular weight

141840Da