AQP1
GeneName
AQP1
Summary
AQP1, also known as aquaporin-1, is a 29kDa integral membrane protein that functions primarily as a water channel, facilitating the transport of water and small solutes across cell membranes. It is expressed in various tissues, including the kidney, where it plays a crucial role in renal water reabsorption, and in the eye, contributing to the formation of aqueous humour. AQP1 is localised to the plasma membrane and is involved in processes such as ammonium and carbon dioxide transport, as well as maintaining cellular homeostasis under varying osmotic conditions. Its diverse functions extend to roles in cellular responses to mechanical stimuli and various signalling pathways, including cGMP-mediated signalling.
Importance
AQP1 is relevant to: - Renal physiology, particularly in the context of water balance and diuretic responses - Eye health, due to its role in aqueous humour dynamics and potential implications in glaucoma - Cancer biology, as it may influence tumour microenvironment through its effects on cell migration and proliferation - Pathophysiological conditions involving cellular hydration and osmotic stress, such as heart failure and diabetes insipidus - Developmental biology, particularly in processes like kidney and eye morphogenesis.
Top Products
For researchers investigating AQP1, we recommend two excellent primary antibodies. The first is the well-cited Anti-Aquaporin 1 antibody [1/22] (ab9566), which has garnered 80 citations and is highly regarded for its performance in immunohistochemistry (IHC), western blotting (WB), and flow cytometry (FC). This antibody is a reliable choice for those looking to study AQP1 in various applications. Additionally, we offer the recombinant antibody Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387), which has been validated in IHC, WB, immunocytochemistry (ICC), and FC. With 59 citations, this recombinant product provides the batch-to-batch consistency that researchers often require. Together, these antibodies provide robust options for studying AQP1 in your research.
Abcam Product Citation Summary
The data indicates that AQP1 antibodies have been effectively used in various immunofluorescence and immunocytochemistry applications across different species, particularly in human and mouse cell types. This suggests a significant role of AQP1 in cellular functions related to stem/progenitor cells and kidney proximal tubular cells, as well as in corneal endothelial cells.
Abcam Product Citation Table
Domain
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Function
Forms a water channel that facilitates the transport of water across cell membranes, playing a crucial role in water homeostasis in various tissues (PubMed:1373524, PubMed:23219802). Could also be permeable to small solutes including hydrogen peroxide, glycerol and gases such as amonnia (NH3), nitric oxide (NO) and carbon dioxide (CO2) (PubMed:16682607, PubMed:17012249, PubMed:19273840, PubMed:33028705, PubMed:8584435). Recruited to the ankyrin-1 complex, a multiprotein complex of the erythrocyte membrane, it could be part of a CO2 metabolon, linking facilitated diffusion of CO2 across the membrane, anion exchange of Cl(-)/HCO3(-) and interconversion of dissolved CO2 and carbonic acid in the cytosol (PubMed:17012249, PubMed:35835865). In vitro, it shows non-selective gated cation channel activity and may be permeable to cations like K(+) and Na(+) in vivo (PubMed:36949749, PubMed:8703053).
Sequence Similarities
Belongs to the MIP/aquaporin (TC 1.A.8) family.
Tissue Specificity
Detected in erythrocytes (at protein level). Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
Cellular localization
- Cell membrane
- Multi-pass membrane protein
Alternative names
CHIP28, AQP1, Aquaporin-1, AQP-1, Aquaporin-CHIP, Channel-like integral membrane protein of 28 kDa, Urine water channel