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Domain

The protein is evolutionarily related to retrotransposon Gag proteins: it contains large N- and C-terminal domains that form a bi-lobar architecture similar to the capsid domain of human immunodeficiency virus (HIV) gag protein. It contains structural elements found within viral Gag polyproteins originated from the Ty3/gypsy retrotransposon family and retains the ability to form virion-like capsid structures that can mediate mRNA transfer between cells. Tetrapod and fly Arc protein-coding genes originated independently from distinct lineages of Ty3/gypsy retrotransposons.

Function

Master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer in the nervous system. ARC protein is released from neurons in extracellular vesicles that mediate the transfer of ARC mRNA into new target cells, where ARC mRNA can undergo activity-dependent translation. ARC capsids are endocytosed and are able to transfer ARC mRNA into the cytoplasm of neurons. Acts as a key regulator of synaptic plasticity: required for protein synthesis-dependent forms of long-term potentiation (LTP) and depression (LTD) and for the formation of long-term memory. Regulates synaptic plasticity by promoting endocytosis of AMPA receptors (AMPARs) in response to synaptic activity: this endocytic pathway maintains levels of surface AMPARs in response to chronic changes in neuronal activity through synaptic scaling, thereby contributing to neuronal homeostasis. Acts as a postsynaptic mediator of activity-dependent synapse elimination in the developing cerebellum by mediating elimination of surplus climbing fiber synapses. Accumulates at weaker synapses, probably to prevent their undesired enhancement. This suggests that ARC-containing virion-like capsids may be required to eliminate synaptic material. Required to transduce experience into long-lasting changes in visual cortex plasticity and for long-term memory (By similarity). Involved in postsynaptic trafficking and processing of amyloid-beta A4 (APP) via interaction with PSEN1 (By similarity). In addition to its role in synapses, also involved in the regulation of the immune system: specifically expressed in skin-migratory dendritic cells and regulates fast dendritic cell migration, thereby regulating T-cell activation (By similarity).

Post-translational modifications

Palmitoylation anchors the protein into the membrane by allowing direct insertion into the hydrophobic core of the lipid bilayer.

Ubiquitinated by UBE3A, leading to its degradation by the proteasome, thereby promoting AMPA receptors (AMPARs) expression at synapses (By similarity). Ubiquitinated by RNF216 at Lys-268 and Lys-269 limiting ARC protein levels induced by synaptic activity and thus regulating ARC-dependent forms of synaptic plasticity (PubMed:24945773).

Phosphorylation at Ser-260 by CaMK2 prevents homooligomerization into virion-like capsids by disrupting an interaction surface essential for high-order oligomerization. Phosphorylation by CaMK2 inhibits synaptic activity.

Sequence similarities

Belongs to the ARC/ARG3.1 family.

Cellular localization

  • Extracellular vesicle membrane
  • Lipid-anchor
  • Postsynaptic cell membrane
  • Lipid-anchor
  • Synapse
  • Postsynaptic density
  • Early endosome membrane
  • Cell projection
  • Dendrite
  • Cytoplasm
  • Cytoskeleton
  • Cytoplasm
  • Cell cortex
  • Cell projection
  • Dendritic spine
  • Cytoplasmic vesicle
  • Secretory vesicle
  • Acrosome
  • Cytoplasmic vesicle
  • Clathrin-coated vesicle membrane
  • Forms virion-like extracellular vesicles that are released from neurons. Enriched in postsynaptic density of dendritic spines. Targeted to inactive synapses following interaction with CAMK2B in the kinase inactive state. Accumulation at weaker synapses may be required to prevent their undesired enhancement. Associated with the cell cortex of neuronal soma and dendrites (By similarity). Associated with the sperm tail (By similarity).

Alternative names

KIAA0278, ARC, Activity-regulated cytoskeleton-associated protein, hArc, Activity-regulated gene 3.1 protein homolog, ARC/ARG3.1, Arg3.1

Target type

Proteins

Primary research area

Neuroscience

Molecular weight

45316Da

We found 8 products in 3 categories

Primary Antibodies

Proteins & Peptides

Target

Species of origin

Cell Lines & Lysates

Target

Cell type

Species or organism

Search our catalogue for 'ARC' (8)

Products

ab183183

Anti-Arc antibody [EPR18950]

Recombinant
RabMAb
KO Validated

ab309193

Anti-Arc antibody [EPR24093-90]

Recombinant
RabMAb
20ul selling size

ab224717

Anti-Arc antibody [EPR18950] - BSA and Azide free

Recombinant
RabMAb
KO Validated

ab280071

Mouse ARC knockout Neuro-2a cell line

Advanced Validation