ARHGAP32
Domain
The N-terminal PX domain interacts specifically with phosphatidylinositides.
Function
GTPase-activating protein (GAP) promoting GTP hydrolysis on RHOA, CDC42 and RAC1 small GTPases. May be involved in the differentiation of neuronal cells during the formation of neurite extensions. Involved in NMDA receptor activity-dependent actin reorganization in dendritic spines. May mediate cross-talks between Ras- and Rho-regulated signaling pathways in cell growth regulation. Isoform 2 has higher GAP activity (By similarity).
Post-translational modifications
Isoform 2 is phosphorylated on multiple tyrosine residues by FYN. Phosphorylated tyrosine residues undergo dephosphorylation after stimulation of NMDA receptors (By similarity). Phosphorylated in vitro by CaMK2 in the presence of calmodulin and calcium; which inhibits GAP activity (By similarity).
Sequence Similarities
Belongs to the PX domain-containing GAP family.
Tissue Specificity
Isoform 1 and isoform 2 are highly expressed in brain and testis. Isoform 1 is also expressed in other tissues such as lung, liver and spleen.
Cellular localization
- Postsynaptic density
- Cell projection
- Dendritic spine
- Cytoplasm
- Cell cortex
- Endosome membrane
- Golgi apparatus membrane
- Endoplasmic reticulum membrane
- Membrane
- Association to membrane via PX domain (PubMed:17663722). Associated with cortical actin in undifferentiated neuroblastoma cells, but localized to dendritic spine and postsynaptic density after differentiation (By similarity). Colocalizes with EGFR at the cell membrane upon EGF treatment (PubMed:12446789). Colocalizes with GAB2 at the cell membrane (PubMed:12819203).
Alternative names
GRIT, KIAA0712, RICS, ARHGAP32, Rho GTPase-activating protein 32, Brain-specific Rho GTPase-activating protein, GAB-associated Cdc42/Rac GTPase-activating protein, GC-GAP, GTPase regulator interacting with TrkA, Rho-type GTPase-activating protein 32, Rho/Cdc42/Rac GTPase-activating protein RICS, RhoGAP involved in the beta-catenin-N-cadherin and NMDA receptor signaling, p200RhoGAP, p250GAP