Aspartate aminotransferase, cytoplasmic
GeneName
GOT1
Summary
GOT1, also known as aspartate aminotransferase, is a 46 kDa enzyme primarily located in the cytoplasm, but also found in the nucleus and axon terminus. It plays a crucial role in amino acid metabolism, particularly in the conversion of L-aspartate and 2-oxoglutarate to L-glutamate and oxaloacetate. This enzyme is involved in various metabolic processes including aspartate biosynthesis and catabolism, gluconeogenesis, and the malate-aspartate shuttle. Additionally, GOT1 is implicated in cellular responses to insulin and mechanical stimuli, highlighting its importance in metabolic regulation and cellular signalling.
Importance
GOT1 is relevant to: - Metabolic disorders due to its central role in amino acid and energy metabolism - Neurobiology, as it is involved in the malate-aspartate shuttle, which is crucial for maintaining neuronal energy homeostasis - Cancer research, given its involvement in the regulation of metabolic pathways that support tumour growth - Cardiovascular health, as it plays a role in fatty acid homeostasis and cellular responses to stress
Top Products
For researchers investigating GOT1, we highly recommend the top-selling recombinant antibody, Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950). This antibody has been validated for use in several applications, including Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), and flow cytometry (FC), making it a versatile tool for your research needs. With 9 citations, it is gaining recognition in the scientific community for its reliability and performance. This recombinant antibody ensures batch-to-batch consistency, providing confidence in your experimental results. The Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881), supported by 5 citations, is an excellent option for researchers looking to accurately measure GOT1 levels in their samples.
Abcam Product Citation Summary
The data indicates that GOT1 is being investigated in the context of metastatic lung cancer in mice and glutamine metabolism in KSHV-transformed cells in rats. This suggests a potential role of GOT1 in cancer biology and metabolic processes.
Abcam Product Citation Table
Function
Biosynthesis of L-glutamate from L-aspartate or L-cysteine (PubMed:21900944). Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In addition, catalyzes (2S)-2-aminobutanoate, a by-product in the cysteine biosynthesis pathway (PubMed:27827456).
Sequence Similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Cellular localization
- Cytoplasm
Alternative names
cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1