ATF3
GeneName
ATF3
Summary
ATF3, also known as A33, ATF-3, or LRF-1, is a 21 kDa transcription factor that plays a vital role in cellular stress responses. It is predominantly expressed in the nucleus and is involved in regulating gene expression in response to various stimuli, including amino acid starvation and endoplasmic reticulum stress. ATF3 functions as both an activator and repressor of transcription, interacting with RNA polymerase II and binding to specific DNA sequences to modulate the expression of target genes. It is found in various subcellular locations, including the nucleus, nucleolus, and centrosome, suggesting diverse roles in cellular processes such as differentiation and proliferation.
Importance
ATF3 is relevant to: - The cellular response to stress, particularly in the context of the endoplasmic reticulum unfolded protein response, which is crucial for maintaining cellular homeostasis. - Regulation of metabolic processes such as gluconeogenesis, impacting energy balance and glucose homeostasis. - Modulation of apoptosis through its positive regulation of TRAIL-activated apoptotic signalling pathways, which has implications in cancer research. - The negative regulation of ERK1 and ERK2 signalling cascades, influencing pathways associated with cell growth and differentiation.
Top Products
For researchers investigating ATF3, we highly recommend the top-selling recombinant monoclonal antibody, Anti-ATF3 antibody [EPR19488] - ChIP Grade (ab207434). This antibody has been validated in knockout models, ensuring reliable performance in various applications, including Western blotting (WB), chromatin immunoprecipitation (ChIP), immunocytochemistry (ICC), and immunoprecipitation (IP). With 63 citations, it is well-regarded in the research community, making it an excellent choice for those seeking dependable ATF3 detection in their studies.
Abcam Product Citation Summary
The data indicates that the ATF3 antibody (ab207434) has been utilised in various studies focusing on pancreatic cancer and intestinal biology. Specifically, it has been employed in western blotting and RNA immunoprecipitation to explore the role of ATF3 in human pancreatic cancer cells and in mouse colon fragments, highlighting its relevance in cancer research and gut health.
Abcam Product Citation Table
Function
This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter.
Isoform 2
Activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
Isoform 3
Stress-induced isoform, counteracts the transcriptional repression of isoform 1.
Sequence Similarities
Belongs to the bZIP family. ATF subfamily.
Cellular localization
- Nucleus
Alternative names
Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3, Activating transcription factor 3, ATF3