BAG3
GeneName
BAG3
Summary
BAG3, also known as bis, is a 62 kDa protein that plays a multifaceted role in cellular processes, particularly in protein homeostasis and stress responses. It is localised in various cellular compartments, including the cytoplasm, nucleus, mitochondria, and aggresomes. BAG3 functions as a chaperone, facilitating protein folding and stabilisation, and is involved in the assembly of aggresomes and autophagosomes. It interacts with multiple proteins through its binding capabilities, including cadherins and dynein intermediate chains, and is implicated in the cellular response to stressors such as heat and mechanical stimuli.
Importance
BAG3 is relevant to: - Chaperone-mediated autophagy and protein quality control, which are essential for cellular health and function - Regulation of apoptosis, particularly in striated muscle cells, influencing muscle cell survival and homeostasis - Mechanisms of neurodegenerative diseases through its role in aggresome formation and the handling of misfolded proteins - Muscle cell development and maintenance, contributing to muscle integrity and function
Top Products
For researchers investigating BAG3, we recommend two excellent primary antibodies that cater to a variety of applications. The first is the well-cited polyclonal antibody, Anti-Bag3 antibody (ab47124), which has garnered 25 citations, reflecting its reliability in Western blotting (WB), immunocytochemistry (ICC), and immunohistochemistry (IHC). This antibody is a trusted choice for those looking to explore BAG3 in different experimental contexts. Additionally, we offer the recombinant antibody, Anti-Bag3 antibody [EPR3515] (ab92309), which has been validated in knockout models and is suitable for Western blotting (WB) and immunoprecipitation (IP). With 20 citations, this monoclonal antibody provides the batch-to-batch consistency that researchers often seek. Together, these antibodies provide robust options for studying BAG3 effectively. The Anti-Bag3 antibody ELISA Kit (ab92309), supported by 20 citations, is an excellent option for researchers looking to accurately measure BAG3 levels in their samples.
Abcam Product Citation Summary
The data indicates that BAG3 is primarily studied in the context of autophagic flux and oxidative stress resistance, particularly in human cell lines. The use of multiple antibodies in Western blotting suggests a focus on validating findings across different experimental setups.
Abcam Product Citation Table
Function
Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70 (PubMed:27474739, PubMed:9873016). Has anti-apoptotic activity (PubMed:10597216). Plays a role in the HSF1 nucleocytoplasmic transport (PubMed:26159920).
Involvement in disease
Myopathy, myofibrillar, 6
MFM6
A form of myofibrillar myopathy, a group of chronic neuromuscular disorders characterized at ultrastructural level by disintegration of the sarcomeric Z disk and myofibrils, and replacement of the normal myofibrillar markings by small dense granules, or larger hyaline masses, or amorphous material. MFM6 is characterized by early-onset of severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients.
None
The disease is caused by variants affecting the gene represented in this entry.
Cardiomyopathy, dilated, 1HH
CMD1HH
A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
None
The disease is caused by variants affecting the gene represented in this entry.
Cellular localization
- Nucleus
- Cytoplasm
- Colocalizes with HSF1 to the nucleus upon heat stress (PubMed:26159920).
Alternative names
BIS, BAG3, BAG family molecular chaperone regulator 3, BAG-3, Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis, Docking protein CAIR-1