Bcar1 phospho Y751
Domain
Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
A serine-rich region promotes activation of the serum response element (SRE).
The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK1.
Function
Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion (By similarity). Implicated in induction of cell migration and cell branching (By similarity). Involved in the BCAR3-mediated inhibition of TGFB signaling (By similarity).
Post-translational modifications
PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin mediated adhesion of cells to the extracellular matrix.
Phosphorylated by SRC kinase in a EDN1- and PTK2B-mediated manner; phosphorylation strengthens its interaction with BCAR3 as part of the PTK2B/BCAR1/BCAR3/RAP1 signaling pathway.
Dephosphorylated by PTPN14 at Tyr-226.
Sequence Similarities
Belongs to the CAS family.
Tissue Specificity
Widely expressed. Higher expression in lung, intestine and testis.
Cellular localization
- Cell junction
- Focal adhesion
- Cytoplasm
- Cell projection
- Axon
- Unphosphorylated form localizes in the cytoplasm (By similarity). Localizes to focal adhesion sites following integrin engagement (By similarity).
Alternative names
Cas, Crkas, Bcar1, Breast cancer anti-estrogen resistance protein 1, CRK-associated substrate, p130cas