BID
Domain
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Function
Induces caspases and apoptosis (PubMed:14583606). Counters the protective effect of BCL2 (By similarity).
BH3-interacting domain death agonist p15
Induces caspase activation and apoptosis (PubMed:15661737, PubMed:32029622). Allows the release of cytochrome c (PubMed:32029622).
Isoform 1
Induces ICE-like proteases and apoptosis.
Isoform 2
Induces ICE-like proteases and apoptosis.
Isoform 3
Does not induce apoptosis.
Isoform 4
Induces ICE-like proteases and apoptosis.
Post-translational modifications
BH3-interacting domain death agonist
TNF-alpha induces caspase-mediated cleavage into a major p15 and minor p13 and p11 products (By similarity). Cleaved by CASP6 into a major p15 and minor p13 products, leading to release of cytochrome c and subsequent nonalcoholic steatohepatitis (PubMed:32029622).
BH3-interacting domain death agonist p15
Ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.
Tissue Specificity
Isoform 2
Expressed in spleen, pancreas and placenta (at protein level).
Isoform 3
Expressed in lung, pancreas and spleen (at protein level).
Isoform 4
Expressed in lung and pancreas (at protein level).
Cellular localization
- Cytoplasm
- Mitochondrion membrane
- Mitochondrion outer membrane
- When uncleaved, it is predominantly cytoplasmic.
- BH3-interacting domain death agonist p15
- Mitochondrion membrane
- Translocates to mitochondria as an integral membrane protein.
- BH3-interacting domain death agonist p13
- Mitochondrion membrane
- Associated with the mitochondrial membrane.
- Isoform 1
- Cytoplasm
- Isoform 3
- Cytoplasm
- Isoform 2
- Mitochondrion membrane
- A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively.
Alternative names
BH3-interacting domain death agonist, p22 BID, BID