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Domain

The C-terminal disordered region undergoes liquid-liquid phase separation (LLPS) for the formation of a membraneless compartment that concentrates mRNAs with associated regulatory factors (PubMed:31439799, PubMed:34074792, PubMed:36040869). CAPRIN1 molecules in the condensed phase are neutral (PubMed:36040869). mRNA-binding promotes phase separation (PubMed:31439799). Moderate concentrations of ATP enhance phase separation by reducing the electrostatic potential of CAPRIN1, thereby promoting intermolecular interactions (PubMed:34074792, PubMed:36040869). In contrast, high concentrations of ATP invert the electrostatic potential of CAPRIN1, so that CAPRIN1 molecules become negatively charged, lead to inhibition of phase separation (PubMed:36040869).

Function

mRNA-binding protein that acts as a regulator of mRNAs transport, translation and/or stability, and which is involved in neurogenesis, synaptic plasticity in neurons and cell proliferation and migration in multiple cell types (PubMed:17210633, PubMed:31439799, PubMed:35979925). Plays an essential role in cytoplasmic stress granule formation (PubMed:35977029). Acts as an mRNA regulator by mediating formation of some phase-separated membraneless compartment: undergoes liquid-liquid phase separation upon binding to target mRNAs, leading to assemble mRNAs into cytoplasmic ribonucleoprotein granules that concentrate mRNAs with associated regulatory factors (PubMed:31439799, PubMed:32302570, PubMed:32302571, PubMed:32302572, PubMed:34074792, PubMed:36040869, PubMed:36279435). Undergoes liquid-liquid phase separation following phosphorylation and interaction with FMR1, promoting formation of cytoplasmic ribonucleoprotein granules that concentrate mRNAs with factors that inhibit translation and mediate deadenylation of target mRNAs (PubMed:31439799). In these cytoplasmic ribonucleoprotein granules, CAPRIN1 mediates recruitment of CNOT7 deadenylase, leading to mRNA deadenylation and degradation (PubMed:31439799). Binds directly and selectively to MYC and CCND2 mRNAs (PubMed:17210633). In neuronal cells, directly binds to several mRNAs associated with RNA granules, including BDNF, CAMK2A, CREB1, MAP2, NTRK2 mRNAs, as well as to GRIN1 and KPNB1 mRNAs, but not to rRNAs (PubMed:17210633).

Involvement in disease

Neurodegeneration, childhood-onset, with cerebellar ataxia and cognitive decline

CONDCAC

A neurodegenerative disorder characterized by early-onset ataxia, dysarthria, cognitive decline, sensorimotor axonal neuropathy and muscle weakness. Brain imaging shows cerebellar atrophy.

None

The disease is caused by variants affecting the gene represented in this entry.

Neurodevelopmental disorder with language impairment, autism, and attention deficit-hyperactivity disorder

NEDLAAD

An autosomal dominant disorder with variable expressivity and incomplete penetrance. It is characterized by language impairment, speech delay, intellectual disability, attention deficit hyperactivity disorder and autism spectrum disorder. Additional variable features include developmental delay, seizures, skeletal anomalies, respiratory difficulties, and ophthalmologic anomalies.

None

The disease is caused by variants affecting the gene represented in this entry.

Post-translational modifications

Tyrosine phosphorylation by EPHA4 promotes interaction with FMR1 and liquid-liquid phase separation (LLPS) for the formation of a membraneless compartment that concentrates mRNAs with associated regulatory factors.

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner (PubMed:22967762). O-glycosylation by OGT inhibit ability to undergo liquid-liquid phase separation (LLPS) (PubMed:34074792).

Sequence similarities

Belongs to the caprin family.

Tissue specificity

Ubiquitous.

Cellular localization

  • Cytoplasm
  • Cytoplasmic ribonucleoprotein granule
  • Cytoplasm
  • Cytosol
  • Cell projection
  • Dendrite
  • Cell projection
  • Lamellipodium
  • Mediates formation and localizes to cytoplasmic ribonucleoprotein membraneless compartments (PubMed:31439799). Associated with RNA granules. At the leading edge of migrating fibroblasts, colocalizes with DDX3X (PubMed:28733330).
  • Cytoplasm
  • Cytosol
  • (Microbial infection) In case of reovirus infection, associates with the outer peripheries of viral factories in a G3BP1 dependent manner.

Alternative names

GPIAP1, GPIP137, M11S1, RNG105, CAPRIN1, Caprin-1, Cell cycle-associated protein 1, Cytoplasmic activation- and proliferation-associated protein 1, GPI-anchored membrane protein 1, GPI-anchored protein p137, Membrane component chromosome 11 surface marker 1, RNA granule protein 105, GPI-p137, p137GPI

Target type

Proteins

Molecular weight

78366Da

We found 3 products in 1 category

Primary Antibodies

Target

Reactive species

Search our catalogue for 'CAPRIN1' (3)

Products

ab313547

Anti-Caprin-1 antibody [RP23040261]

Recombinant
Advanced Validation