Catalase
GeneName
CAT
Summary
CAT, or catalase, is a 60 kDa enzyme that plays a vital role in the cellular detoxification of hydrogen peroxide, a potentially harmful byproduct of metabolic processes. It is primarily located in the peroxisome, where it catalyses the decomposition of hydrogen peroxide into water and oxygen, thus protecting cells from oxidative damage. In addition to its peroxisomal presence, CAT can also be found in the cytoplasm, mitochondria, and extracellular regions, highlighting its versatile role in various cellular environments. The enzyme exhibits antioxidant activity and is involved in several biological processes, including aerobic respiration, cholesterol metabolism, and the regulation of apoptotic pathways. It binds heme and has multiple enzymatic functions, including oxidoreductase activity.
Importance
CAT is relevant to: - The study of oxidative stress and its implications in various diseases, including cancer and neurodegenerative disorders, due to its role in hydrogen peroxide detoxification. - Research on cellular responses to environmental stressors, such as heavy metals and xenobiotics, given its involvement in detoxification pathways. - Investigations into metabolic disorders, particularly those affecting cholesterol and triglyceride metabolism, as it plays a role in these processes. - Understanding the mechanisms of apoptosis and cell division regulation, which are crucial in cancer research and developmental biology.
Top Products
For researchers investigating catalase (CAT), we recommend two excellent primary antibodies that cater to a variety of applications. The first is the well-cited polyclonal antibody, Anti-Catalase antibody - Peroxisome Marker (ab52477), which has garnered 47 citations and is validated for use in Western blotting (WB) and immunohistochemistry (IHC). Its knockout validation further enhances its reliability for your studies. Additionally, we offer the recombinant monoclonal antibody, Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211). This antibody is also validated in knockout models and is suitable for a broader range of applications, including WB, immunocytochemistry (ICC), and flow cytometry (FC). With 34 citations, it demonstrates a solid presence in the research community. Both antibodies provide robust options for reliable detection of catalase, ensuring that you have the tools necessary for your research. The Catalase Activity Assay Kit (Immunocapture) (ab118184), supported by 12 citations, is an excellent option for researchers looking to measure catalase activity with confidence.
Abcam Product Citation Summary
The data indicates that CAT (catalase) is being studied in various contexts, particularly focusing on oxidative stress and inflammation. The use of Abcam antibodies in Western blotting and immunofluorescence highlights the importance of catalase in different species, including humans and mice, as well as in specific tissues such as fibroblasts, hearts, and tumour tissues. This suggests a significant role for catalase in cellular responses to oxidative stress and related pathologies.
Abcam Product Citation Table
Function
Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide (PubMed:7882369). Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells (PubMed:7882369).
Involvement in disease
Acatalasemia
ACATLAS
A metabolic disorder characterized by a total or near total loss of catalase activity in red cells. It is often associated with ulcerating oral lesions. Acatalasemia is inherited as an autosomal recessive trait.
None
The disease is caused by variants affecting the gene represented in this entry.
Sequence Similarities
Belongs to the catalase family.
Cellular localization
- Peroxisome matrix
Alternative names
Catalase, CAT