CCL5
GeneName
CCL5
Summary
CCL5, also known as RANTES or CCL-5, is a 10 kDa chemokine that is secreted into the extracellular region and plays a pivotal role in immune responses. It is produced by various cell types, including T cells, platelets, and macrophages, and is involved in chemotaxis, guiding the migration of immune cells such as monocytes, eosinophils, and lymphocytes to sites of inflammation. CCL5 binds to several chemokine receptors, including CCR1, CCR4, and CCR5, and is crucial for cell-cell signalling and the regulation of immune cell activities during inflammatory responses and infections.
Importance
CCL5 is relevant to: - The recruitment of immune cells during inflammatory responses, which is vital for understanding autoimmune diseases and chronic inflammation. - Its role in HIV pathogenesis, as it can act as a ligand for CCR5, a co-receptor used by HIV to enter host cells. - Cancer research, particularly in the context of tumour microenvironments, where CCL5 influences the migration and activation of immune cells. - The regulation of viral infections, given its involvement in the immune response to viral pathogens and its ability to modulate viral replication.
Top Products
For researchers investigating CCL5, we recommend two primary antibodies that cater to different experimental needs. The first is the well-cited polyclonal antibody, Anti-RANTES antibody (ab9679), which has garnered 32 citations, highlighting its reliability in ELISA applications. This antibody is a trusted choice for those focusing on quantifying CCL5 levels. Additionally, we offer the recombinant antibody, Anti-RANTES antibody [EPR26519-16] (ab307712), which is suitable for immunohistochemistry (IHC) and western blotting (WB). With 3 citations, this monoclonal antibody provides the batch-to-batch consistency that researchers often seek in their experiments. Together, these antibodies provide a comprehensive toolkit for studying CCL5 effectively. The Recombinant human RANTES protein (Active) ELISA Kit (ab269212) is a reliable option for researchers looking to measure CCL5 in their experiments.
Abcam Product Citation Summary
The data indicates that CCL5 is being studied in various contexts, particularly in relation to inflammation and prostate conditions. The use of antibodies in human samples and different species highlights the relevance of CCL5 in both human health and disease models.
Abcam Product Citation Table
Function
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) (PubMed:1380064, PubMed:15923218, PubMed:16791620, PubMed:8525373, PubMed:9516414). May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells (PubMed:23979485).
Post-translational modifications
N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
N-terminal processed form RANTES(4-68) is produced by proteolytic cleavage by cathepsin CTSG.
The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
Sequence Similarities
Belongs to the intercrine beta (chemokine CC) family.
Tissue Specificity
Expressed in the follicular fluid (at protein level). T-cell and macrophage specific.
Cellular localization
- Secreted
Alternative names
D17S136E, SCYA5, CCL5, C-C motif chemokine 5, EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, T-cell-specific protein RANTES, TCP228
Database links
swissprot:P13501 entrezGene:6352 omim:187011
Other research areas
- Immuno-oncology