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CCNA2

GeneName

CCNA2

Summary

CCNA2, also known as cyclin A or cyclin A2, is a 49kDa protein that plays a vital role in regulating the cell cycle, particularly the G1/S phase transition and the G2/M transition. It forms complexes with cyclin-dependent kinases (CDK1 and CDK2), facilitating their activity in driving cell division and ensuring proper progression through the cell cycle. CCNA2 is expressed in various tissues, localised in the cytoplasm, nucleoplasm, and at the microtubule organising centre, and is involved in a range of cellular responses to hormonal and growth factor stimuli, as well as in processes like animal organ regeneration and cochlea development.

Importance

CCNA2 is relevant to: - Understanding cell cycle regulation and its implications in cancer biology due to its role in cell division and proliferation - Investigating cellular responses to various stimuli, which can inform therapeutic strategies for metabolic disorders and hormonal imbalances - Exploring its involvement in organ regeneration and developmental biology, providing insights into tissue repair mechanisms - Researching the molecular mechanisms underlying diseases linked to dysregulation of the cell cycle, including various cancers and developmental disorders

Top Products

For researchers investigating CCNA2, we highly recommend the top-selling recombinant antibody, Anti-Cyclin A2 antibody [EPR17351] (ab181591). This well-cited product has garnered 130 citations, reflecting its strong reputation in the field. It has been validated for use in several applications, including Western blotting (WB), immunocytochemistry (ICC), and immunohistochemistry (IHC), making it a versatile choice for your research needs. The recombinant nature of this antibody ensures batch-to-batch consistency, providing reliable results in your experiments. The Recombinant Human Cyclin A2 protein ELISA Kit (ab126696), supported by 1 citation, is an excellent option for researchers looking to accurately measure CCNA2 levels in their samples.

Abcam Product Citation Summary

The data indicates that CCNA2 is frequently studied in the context of various cancers, particularly hepatocellular carcinoma (HCC) and acute myeloid leukaemia (AML). The use of Abcam antibodies in Western blotting across different species, including humans and mice, highlights the importance of CCNA2 in cell cycle regulation and proliferation. Additionally, studies involving miR-34a and lncRNA NR-104098 suggest a regulatory role of CCNA2 in cancer progression and response to treatments.

Abcam Product Citation Table

ab181591
Rat
WB
MCT-induced proliferation
31200778
ab181591
Human
WB
Targeting of Cyclin A2 by miR-34a
30546088
ab181591
Human
WB
Deubiquitination regulation
30546088
ab181591
Human
WB
miR-34a/USP7/Cyclin A2 pathway
30546088
ab181591
Human
WB
miR-29c-3p and CCNA2 expression
32154226
ab181591
Mouse
WB
Cell cycle progression after psoralen treatment
30459602
ab181591
Mouse
WB
AML cell differentiation
32296698
ab181591
Human
WB
Cell cycle
31804459
ab181591
Mouse
WB
Hepatic activation after partial hepatectomy
30546054
ab185619
Human
WB
Cell cycle regulation
32392178
ab32498
Human
WB
Cell cycle regulation
24289849
ab38
Mouse
WB
Cardiac response to injury
28053183
ab38
Human
WB
Radioresistance
23886499
ab38
Human
WB
NSCLC cell proliferation
30935108
ab38
Human
WB
Cell-cycle regulation
31261874

Developmental stage

Accumulates steadily during G2 and is abruptly destroyed at mitosis. Not detected during the G1 phase of the cell cycle. It accumulates during the DNA synthesis/S phase and disappears as cells progress into mitosis, between prophase and metaphase (at protein level).

Function

Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.

Post-translational modifications

Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome (PubMed:21596315). Deubiquitinated and stabilized by USP37 enables entry into S phase (PubMed:21596315). Ubiquitinated during the G1 phase by the SCF(FBXO31) complex, leading to its proteasomal degradation (PubMed:31413110).

Sequence Similarities

Belongs to the cyclin family. Cyclin AB subfamily.

Cellular localization

Alternative names

CCN1, CCNA, CCNA2, Cyclin-A2, Cyclin-A, Cyclin A

swissprot:P20248 entrezGene:983 entrezGene:890 swissprot:P24941 swissprot:P06493 omim:123835 omim:116953 omim:116940 entrezGene:1017

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