CCNB1
GeneName
CCNB1
Summary
CCNB1, also known as cyclin B1 or Ccn2, is a 48 kDa protein that plays a pivotal role in cell cycle regulation, particularly during the G2/M transition. It is predominantly expressed in the cytoplasm and nucleus, and is associated with various cellular components including the centrosome, microtubule organizing center, and the cyclin B1-CDK1 complex. CCNB1 functions as an activator and regulator of cyclin-dependent protein serine/threonine kinases, facilitating processes such as mitotic spindle organization and chromosome alignment during cell division. Additionally, it is involved in cellular responses to various stimuli, including hypoxia and fatty acids, and contributes to developmental processes such as oocyte maturation and cardiac muscle cell proliferation.
Importance
CCNB1 is relevant to: - Understanding cell cycle dynamics and its implications in cancer biology, as dysregulation of cyclins is often associated with tumorigenesis - Investigating mechanisms of tissue regeneration and development, particularly in cardiac and embryonic contexts - Exploring the roles of cyclins in response to environmental stressors and xenobiotic stimuli, which may inform therapeutic strategies - Studying the regulation of mitotic processes, which is crucial for maintaining genomic stability and preventing aneuploidy
Top Products
For researchers investigating CCNB1, we highly recommend the top-selling recombinant antibody, Anti-Cyclin B1 antibody [Y106] (ab32053). This well-cited product has garnered 236 citations, reflecting its strong reputation in the field. It has been validated in knockout models and is suitable for a variety of applications, including Western blotting (WB), immunocytochemistry (ICC), immunohistochemistry (IHC), immunoprecipitation (IP), and flow cytometry (FC). This versatility makes it an excellent choice for those seeking reliable detection of CCNB1 across different experimental setups.
Abcam Product Citation Summary
The data indicates that CCNB1 is frequently studied in the context of cell cycle regulation and cancer, particularly in human cell lines. Various studies have utilised Abcam antibodies to investigate the effects of different treatments and genetic modifications on CCNB1 expression, highlighting its role in processes such as apoptosis and cell cycle checkpoints.
Abcam Product Citation Table
Developmental stage
Accumulates steadily during G2 and is abruptly destroyed at mitosis.
Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Post-translational modifications
Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction (PubMed:16009132). Deubiquitinated by USP22 during G2/M phase (PubMed:27030811).
Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.
Sequence Similarities
Belongs to the cyclin family. Cyclin AB subfamily.
Cellular localization
- Cytoplasm
- Nucleus
- Cytoplasm
- Cytoskeleton
- Microtubule organizing center
- Centrosome
Alternative names
CCNB, CCNB1, G2/mitotic-specific cyclin-B1
Database links
swissprot:P14635 entrezGene:891 omim:123836
Other research areas
- Epigenetics