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CD274

Function

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813410, PubMed:28813417, PubMed:31399419). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813410, PubMed:28813417, PubMed:36727298). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077). Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis (PubMed:32929201).

The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813410, PubMed:28813417). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).

Involvement in disease

Truncation of the 3'-untranslated (3'-UTR) region of CD274 transcripts leads to elevated expression of CD274 in multiple cancers including T-cell leukemia, diffuse large B-cell lymphoma and stomach adenocarcinoma (PubMed:27281199). Disruption of 3'-UTR region is caused by structural variants that stabilize CD274 transcripts, leading to overexpression (PubMed:27281199). Increased expression in tumors promotes immune evasion and tumor cell growth by allowing malignant cells to escape destruction by the immune system (PubMed:27281199).

Post-translational modifications

Ubiquitinated; STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation (PubMed:28813410). Ubiquitinated by MARCHF8; leading to degradation (PubMed:34183449). Deubiquitinated by USP22; leading to stabilization (PubMed:31399419).

Sequence similarities

Belongs to the immunoglobulin superfamily. BTN/MOG family.

Tissue specificity

Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.

Isoform 4

Widely expressed, highest in lung, liver and pituitary and in various peripheral blood cells, including neutrophils and some subtypes of lymphoid and myeloid cells.

Cellular localization

  • Cell membrane
  • Single-pass type I membrane protein
  • Early endosome membrane
  • Single-pass type I membrane protein
  • Recycling endosome membrane
  • Single-pass type I membrane protein
  • Nucleus
  • Associates with CMTM6 at recycling endosomes, where it is protected from being targeted for lysosomal degradation (PubMed:28813417). Translocates to the nucleus in response to hypoxia via its interaction with phosphorylated STAT3 (PubMed:32929201).
  • Isoform 1
  • Cell membrane
  • Single-pass type I membrane protein
  • Isoform 2
  • Endomembrane system
  • Single-pass type I membrane protein
  • Isoform 4
  • Secreted

Alternative names

  • B7H1
  • PDCD1L1
  • PDCD1LG1
  • PDL1
  • B7H1
  • PDL1
  • PDCD1LG1
  • PDCD1L1
  • CD274
  • Programmed cell death 1 ligand 1
  • PD-L1
  • PDCD1 ligand 1
  • Programmed death ligand 1
  • hPD-L1
  • B7 homolog 1
  • B7-H1

Target type

Proteins

Primary research area

Immuno-oncology

Other research areas

  • Immunology & Infectious Disease

Molecular weight

33275Da

We found 10 products in 3 categories

Assay Kits

Target

Reactive species

Detection method

Proteins & Peptides

Target

Species of origin