CD63
GeneName
CD63
Summary
CD63, also known as LAMP3, granulophysin, or LAMP-3, is a 26kDa transmembrane protein predominantly expressed in various immune cells, including granulocytes and platelets. It is localised to several cellular compartments such as the plasma membrane, endosomes, lysosomes, and extracellular exosomes. CD63 plays a role in cell migration and adhesion, influencing processes like epithelial cell differentiation and integrin-mediated signalling. It is involved in the transport of proteins and the maturation of pigment granules, contributing to the regulation of vascular endothelial growth factor signalling and potassium ion transport.
Importance
CD63 is relevant to: - Immune response and inflammation due to its expression in immune cells and involvement in cell migration - Cancer research, as it may influence tumour cell adhesion and metastasis - Understanding lysosomal function and intracellular transport mechanisms - Studies on cell differentiation and developmental biology, particularly in epithelial tissues
Top Products
For researchers investigating CD63, we highly recommend the top-selling recombinant antibody, Anti-CD63 antibody [EPR5702] (ab134045). This well-cited product has garnered 624 citations, reflecting its strong reputation in the field. It has been validated in knockout models and is suitable for a variety of applications, including Western blotting (WB) and immunohistochemistry (IHC). The reliability and consistency of this recombinant antibody make it an excellent choice for those studying the late endosome marker CD63. The Anti-CD63 antibody [EPR5702] - Late Endosome Marker ELISA Kit (ab134045), with an impressive 624 citations, is an excellent option for researchers looking to accurately measure CD63 levels in their experiments.
Abcam Product Citation Summary
The data indicates a significant focus on the use of CD63 antibodies in various studies related to exosomes and extracellular vesicles across multiple species, particularly in human and mouse models. The applications primarily involve Western Blotting and Immunohistochemistry, highlighting the importance of CD63 as a marker in studies of cancer, aging, and cellular processes. The research spans a range of contexts, including age-related macular degeneration, cancer stemness, and the characterization of exosomes derived from different cell types.
Abcam Product Citation Table
Function
The protein expressed by gene CD63 functions as a cell surface receptor for TIMP1 and is involved in the activation of various cellular signaling cascades. It plays a role in the activation of ITGB1 and integrin signaling, which leads to the activation of AKT, FAK/PTK2, and MAP kinases, promoting cell survival, actin cytoskeleton reorganization, and cell adhesion, spreading, and migration through the activation of AKT and FAK/PTK2. Additionally, it participates in VEGFA signaling by regulating the internalization of KDR/VEGFR2. CD63 is involved in intracellular vesicular transport processes and is essential for the normal trafficking of the PMEL luminal domain, crucial for melanocyte development and maturation. It also contributes to leukocyte adhesion onto endothelial cells by regulating SELP trafficking. CD63 may play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in response to other stimuli. This supplementary information is collated from multiple sources and compiled automatically.
Post-translational modifications
Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro).
Sequence Similarities
Belongs to the tetraspanin (TM4SF) family.
Tissue Specificity
Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
Cellular localization
- Cell membrane
- Multi-pass membrane protein
- Lysosome membrane
- Multi-pass membrane protein
- Late endosome membrane
- Multi-pass membrane protein
- Endosome
- Multivesicular body
- Melanosome
- Secreted
- Extracellular exosome
- Cell surface
- Also found in Weibel-Palade bodies of endothelial cells (PubMed:10793155). Located in platelet dense granules (PubMed:7682577). Detected in a subset of pre-melanosomes. Detected on intralumenal vesicles (ILVs) within multivesicular bodies (PubMed:21962903).
Alternative names
CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, LAMP-3, Limp1, Tspan-30