CDH1
GeneName
CDH1
Summary
CDH1, also known as E-cadherin or L-CAM, is a 97kDa transmembrane glycoprotein that plays a crucial role in cell-cell adhesion, primarily within epithelial tissues. It is localised to various cellular components including adherens junctions, desmosomes, and the lateral plasma membrane. CDH1 mediates calcium-dependent adhesion between cells, facilitating the organisation of adherens junctions and contributing to cellular processes such as morphogenesis and migration. Additionally, it interacts with catenins and other proteins to form complexes that are essential for maintaining tissue architecture and integrity.
Importance
CDH1 is relevant to: - Tumour suppression, as loss of E-cadherin function is associated with increased invasiveness and metastasis in cancers, particularly gastric and breast cancer. - Developmental biology, due to its role in cell adhesion and organisation during embryogenesis. - Neurobiology, as it influences neuron projection development and synaptic assembly. - Tissue engineering and regenerative medicine, where understanding cadherin-mediated adhesion can inform the design of biomaterials and cell therapies.
Top Products
For researchers investigating CDH1, we recommend two excellent primary antibodies that cater to a variety of applications. The first is the highly regarded Anti-E Cadherin antibody [EP700Y] (ab40772), which has garnered 1059 citations, underscoring its reliability in the field. This monoclonal antibody is validated for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), and flow cytometry (FC), making it a versatile choice for your research needs. Importantly, it has also been validated in knockout models, ensuring its effectiveness in specific experimental contexts.Additionally, we offer the recombinant antibody, Anti-E Cadherin antibody [SP64] (ab227639). This product is also validated in knockout models and is suitable for WB and IHC applications. With 28 citations, it demonstrates a solid presence in the research community. The recombinant nature of this antibody provides batch-to-batch consistency, which is essential for reproducible results. Together, these antibodies provide robust options for studying CDH1 effectively. The Human E-Cadherin ELISA Kit (ab233611), supported by 17 citations, is an excellent option for researchers looking to accurately measure CDH1 levels in their samples.
Abcam Product Citation Summary
The data indicates that CDH1 (E-cadherin) is frequently studied in various human cancer contexts, particularly in relation to cell migration, invasion, and epithelial-mesenchymal transition (EMT). The use of Abcam antibodies in Western blotting and immunohistochemistry highlights the importance of CDH1 in understanding cancer progression and metastasis across different types of cancers, including glioma, liver cancer, and breast cancer.
Abcam Product Citation Table
Domain
Three calcium ions are usually bound at the interface of each cadherin domain and strengthen the connections, imparting a strong curvature to the full-length ectodomain.
Function
Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques (PubMed:29999492). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
(Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria.
Involvement in disease
Diffuse gastric and lobular breast cancer syndrome
DGLBC
A cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. In addition to gastric cancer, most female mutation carriers develop lobular carcinoma of the breast.
None
Disease susceptibility is associated with variants affecting the gene represented in this entry. Heterozygous CDH1 germline mutations are responsible for familial cases of diffuse gastric cancer. Somatic mutations has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
Endometrial cancer
ENDMC
A malignancy of endometrium, the mucous lining of the uterus. Most endometrial cancers are adenocarcinomas, cancers that begin in cells that make and release mucus and other fluids.
None
Disease susceptibility is associated with variants affecting the gene represented in this entry.
Ovarian cancer
OC
The term ovarian cancer defines malignancies originating from ovarian tissue. Although many histologic types of ovarian tumors have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
None
Disease susceptibility is associated with variants affecting the gene represented in this entry.
Breast cancer, lobular
LBC
A type of breast cancer that begins in the milk-producing glands (lobules) of the breast.
None
The gene represented in this entry may be involved in disease pathogenesis.
Blepharocheilodontic syndrome 1
BCDS1
A form of blepharocheilodontic syndrome, a rare autosomal dominant disorder. It is characterized by lower eyelid ectropion, upper eyelid distichiasis, euryblepharon, bilateral cleft lip and palate, and features of ectodermal dysplasia, including hair anomalies, conical teeth and tooth agenesis. An additional rare manifestation is imperforate anus. There is considerable phenotypic variability among affected individuals.
None
The disease is caused by variants affecting the gene represented in this entry.
Post-translational modifications
During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 (PubMed:10597309, PubMed:11076937, PubMed:11953314). Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm (PubMed:10597309). The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway (PubMed:10597309). Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system (PubMed:11076937). The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions (PubMed:11953314). During development of the cochlear organ of Corti, cleavage by ADAM10 at adherens junctions promotes pillar cell separation (By similarity).
N-glycosylation at Asn-637 is essential for expression, folding and trafficking. Addition of bisecting N-acetylglucosamine by MGAT3 modulates its cell membrane location (PubMed:19403558).
Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754.
O-glycosylated. O-manosylated by TMTC1, TMTC2, TMTC3 or TMTC4. Thr-285 and Thr-509 are O-mannosylated by TMTC2 or TMTC4 but not TMTC1 or TMTC3.
(Microbial infection) Cleaved by S.pyogenes SpeB protease; leading to its degradation (PubMed:23532847). Degradation by SpeB promotes bacterial translocation across the host epithelial barrier (PubMed:23532847).
Tissue Specificity
Expressed in granuloma macrophages (at protein level) (PubMed:27760340). Expressed in the skin (at protein level) (PubMed:22294297). Expressed in the liver (PubMed:3263290).
Cellular localization
- Cell junction
- Adherens junction
- Cell membrane
- Single-pass type I membrane protein
- Endosome
- Golgi apparatus
- trans-Golgi network
- Cytoplasm
- Cell junction
- Desmosome
- Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane. Recruited to desmosomes at the initial assembly phase and also accumulates progressively at mature desmosome cell-cell junctions (PubMed:25208567, PubMed:29999492). Localizes to cell-cell contacts as keratinocyte differentiation progresses (By similarity).
Alternative names
CD324, CDHE, UVO, CDH1, Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin
Database links
swissprot:P12830 entrezGene:1014 omim:114021 omim:192090 omim:603006 entrezGene:1015 entrezGene:1002 entrezGene:1012 entrezGene:1009 entrezGene:1008 entrezGene:1006 entrezGene:1005 entrezGene:1004 entrezGene:1003 omim:114020 entrezGene:1001 entrezGene:999 swissprot:P55283 swissprot:P33151 swissprot:P22223 swissprot:P19022 omim:601120
Other research areas
- Neuroscience