CDH2
GeneName
CDH2
Summary
CDH2, also known as N cadherin or NCAD, is a 100kDa transmembrane protein that plays a crucial role in cell-cell adhesion, particularly in the formation and maintenance of adherens junctions. It is widely expressed in various tissues, including the nervous system, heart, and epithelial cells. CDH2 is involved in multiple biological processes such as brain morphogenesis, blood vessel morphogenesis, and cell migration. Its function is mediated through calcium-dependent interactions with other cadherins and catenins, facilitating the assembly of cell junctions and contributing to tissue integrity and cellular organisation.
Importance
CDH2 is relevant to: - Developmental biology, particularly in processes like brain and heart development due to its role in cell adhesion and tissue morphogenesis. - Cancer research, as altered expression of CDH2 is associated with epithelial-to-mesenchymal transition (EMT), influencing tumour progression and metastasis. - Neurobiology, given its involvement in neuronal cell adhesion and synapse assembly, impacting neural circuit formation and function. - Cardiovascular research, as it is essential for maintaining cardiac tissue architecture and function.
Top Products
For researchers investigating CDH2, we recommend two excellent primary antibodies. The first is the highly regarded polyclonal antibody, Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203), which has garnered an impressive 985 citations, reflecting its reliability and trust within the scientific community. This antibody is versatile, suitable for applications including Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), and immunoprecipitation (IP).Additionally, we offer the recombinant antibody, Anti-N Cadherin antibody [EPR1791-4] (ab76011), which has been validated in knockout models and is ideal for WB and IHC applications. With 606 citations, this monoclonal antibody provides the batch-to-batch consistency that researchers often seek. Together, these antibodies represent a robust toolkit for studying CDH2 effectively. The Anti-pan Cadherin antibody [mAbcam22744] ELISA Kit (ab22744), supported by 27 citations, is an excellent option for researchers looking to measure CDH2 levels with confidence.
Abcam Product Citation Summary
The data indicates that CDH2 is frequently studied in the context of epithelial-mesenchymal transition (EMT) across various human cancer types, including lung, breast, and colorectal cancers. The use of Abcam antibodies in Western blotting and immunohistochemistry highlights the importance of CDH2 in cancer cell migration, invasion, and tumor progression. Additionally, studies involving mouse models suggest a broader relevance of CDH2 in understanding EMT mechanisms.
Abcam Product Citation Table
Domain
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. Calcium-binding sites are occupied sequentially in the order of site 3, then site 2 and site 1.
Function
Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).
Involvement in disease
Arrhythmogenic right ventricular dysplasia, familial, 14
ARVD14
A congenital heart disease characterized by infiltration of adipose and fibrous tissue into the right ventricle and loss of myocardial cells, resulting in ventricular and supraventricular arrhythmias.
None
The disease may be caused by variants affecting the gene represented in this entry.
Agenesis of corpus callosum, cardiac, ocular, and genital syndrome
ACOGS
An autosomal dominant, syndromic neurodevelopmental disorder characterized by global developmental delay and/or intellectual disability, corpus callosum agenesis or hypoplasia, mirror movements, dysmorphic features, and ocular, cardiac, and genital anomalies.
None
The disease is caused by variants affecting the gene represented in this entry.
Attention deficit-hyperactivity disorder 8
ADHD8
A form of attention deficit-hyperactivity disorder, a neurobehavioral developmental condition primarily characterized by the coexistence of attentional problems and hyperactivity, with each feature occurring infrequently alone. ADHD8 is an autosomal recessive form with onset in early childhood, usually by age 3 years. ADHD8 patients may manifest mild developmental delay with autism.
None
The disease is caused by variants affecting the gene represented in this entry.
Post-translational modifications
Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa N-terminal soluble fragment and a 45 kDa membrane-bound C-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa (By similarity). Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).
May be phosphorylated by OBSCN.
Cellular localization
- Cell membrane
- Single-pass type I membrane protein
- Cell membrane
- Sarcolemma
- Cell junction
- Cell surface
- Cell junction
- Desmosome
- Cell junction
- Adherens junction
- Cell projection
- Axon
- Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes.
Alternative names
CD325, CDHN, NCAD, CDH2, Cadherin-2, CDw325, Neural cadherin, N-cadherin
Database links
swissprot:P19022 entrezGene:1009 omim:192090 entrezGene:1015 entrezGene:1004 omim:603006 omim:601120 swissprot:P22223 swissprot:P12830 swissprot:P33151 swissprot:P55283 omim:114021 omim:114020 entrezGene:999 entrezGene:1000 entrezGene:1001 entrezGene:1002 entrezGene:1003 entrezGene:1014 entrezGene:1005 entrezGene:1006 entrezGene:1008 entrezGene:1012