CDH5
GeneName
CDH5
Summary
CDH5, also known as VE cadherin or vascular endothelial cadherin, is an 88 kDa transmembrane protein primarily expressed in endothelial cells. It plays a vital role in maintaining the integrity of adherens junctions and tight junctions between endothelial cells, facilitating cell-cell adhesion and communication. CDH5 is involved in several biological processes, including blood vessel maturation, endothelial cell morphogenesis, and the maintenance of the blood-brain barrier. Its function is mediated through various interactions, including binding to beta-catenin and vascular endothelial growth factor receptor 2, which are crucial for signalling pathways that regulate angiogenesis and vascular permeability.
Importance
CDH5 is relevant to: - Angiogenesis and vascular development, as it regulates endothelial cell adhesion and migration during blood vessel formation - Maintenance of the blood-brain barrier, contributing to neurological health and disease - Inflammatory responses, where it plays a role in modulating endothelial permeability - Cancer biology, particularly in tumour angiogenesis and metastasis, due to its influence on endothelial cell behaviour and vascular integrity
Top Products
For researchers investigating CDH5, we recommend two excellent primary antibodies. The first is the well-cited polyclonal antibody, Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168), which has garnered 477 citations, highlighting its reliability in Western blotting (WB) and immunocytochemistry (ICC). Additionally, we offer the recombinant antibody, Anti-VE Cadherin antibody [EPR27436-55] (ab313632). This versatile monoclonal antibody has been validated for use in a broader range of applications, including immunohistochemistry (IHC), flow cytometry (FC), and immunoprecipitation (IP), making it an excellent choice for researchers seeking consistency and performance across various experimental setups. The Anti-VE Cadherin antibody ELISA Kit (ab232880), supported by 15 citations, is an excellent option for researchers looking to measure CDH5 with confidence.
Abcam Product Citation Summary
The data indicates a significant focus on the role of CDH5 in various endothelial contexts, particularly in human and mouse models. Studies often utilise Western blotting and immunofluorescence to explore CDH5's involvement in adhesion properties, calcium signalling, and endothelial-to-mesenchymal transition (EndMT). The research spans multiple species, including humans, mice, and rats, highlighting the importance of CDH5 in vascular biology and related pathologies.
Abcam Product Citation Table
Domain
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
Function
Cadherins are calcium-dependent cell adhesion proteins (By similarity). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (PubMed:21269602). This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions (By similarity). It associates with alpha-catenin forming a link to the cytoskeleton (PubMed:10861224). Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1 (By similarity). Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen (By similarity). These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B (PubMed:20332120). Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction (PubMed:20332120).
Post-translational modifications
Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB (By similarity).
O-glycosylated.
Tissue Specificity
Endothelial tissues and brain.
Cellular localization
- Cell junction
- Adherens junction
- Cell membrane
- Single-pass type I membrane protein
- Cytoplasm
- Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions.
Alternative names
CD144, Cadherin-5, 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CDH5