CDX2
GeneName
CDX2
Summary
CDX2, also known as CDX-2 or homeobox protein CDX2, is a 34 kDa transcription factor predominantly expressed in the intestinal epithelium and plays a crucial role in the regulation of gene expression during development. It is localised in the nucleus and is involved in various biological processes such as intestinal epithelial cell differentiation, blood vessel development, and the establishment of epithelial cell polarity. CDX2 functions as a DNA-binding transcription factor, specifically regulating RNA polymerase II-mediated transcription and is implicated in morphogenetic processes and the maintenance of stem cell populations.
Importance
CDX2 is relevant to: - Intestinal development and homeostasis, as it regulates the differentiation of intestinal epithelial cells and maintains the integrity of the intestinal barrier - Cancer research, particularly colorectal cancer, where CDX2 expression is often altered and serves as a potential biomarker - Understanding organ morphogenesis and developmental biology due to its role in anterior/posterior axis specification and organ formation - Stem cell biology, as it influences stem cell differentiation and population maintenance in various tissues.
Top Products
For researchers investigating CDX2, we recommend two primary antibodies that stand out for their reliability and performance. The first is the well-cited monoclonal antibody, Anti-CDX2 antibody [EPR2764Y] (ab76541), which has garnered 166 citations, highlighting its trustworthiness in the field. This antibody is particularly effective for immunohistochemistry (IHC), immunocytochemistry (ICC), and western blotting (WB), making it a versatile choice for various applications.In addition, we offer the recombinant antibody, Anti-CDX2 antibody [SP54] (ab101532). This product is validated for IHC, ICC, and flow cytometry (FC), providing researchers with the batch-to-batch consistency that recombinant antibodies are known for. With its proven performance, this antibody is an excellent option for those studying CDX2.
Abcam Product Citation Summary
The data indicates that the CDX2 antibody (ab76541) has been extensively used in various studies involving both mouse and human models. The applications primarily include immunohistochemistry and immunofluorescence, highlighting its role in understanding differentiation processes in embryonic stem cells, histological alterations in colorectal cancer, and intestinal identity post-injury. This suggests that CDX2 is a significant marker in developmental biology and cancer research.
Abcam Product Citation Table
Function
Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium (By similarity). Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription (By similarity). Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription (By similarity). Binds to and activates transcription of LPH (By similarity). Activates transcription of CLDN2 and intestinal mucin MUC2 (By similarity). Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription (By similarity). Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA (PubMed:28473536).
Post-translational modifications
Ubiquitinated, leading to its degradation by the proteasome.
Phosphorylation at Ser-60 reduces transactivation capacity. Phosphorylation at Ser-283 reduces transactivation capacity and also increases ubiquitin-dependent proteasome degradation.
Sequence Similarities
Belongs to the Caudal homeobox family.
Tissue Specificity
Detected in small intestine, colon and pancreas.
Cellular localization
- Nucleus
Alternative names
CDX3, CDX2, Homeobox protein CDX-2, CDX-3, Caudal-type homeobox protein 2