CFL1
GeneName
CFL1
Summary
CFL1, also known as cofilin or cofilin-1, is a 19 kDa actin-binding protein that plays a crucial role in the regulation of the actin cytoskeleton. It is predominantly expressed in neuronal tissues, where it is involved in processes such as dendritic spine development and synaptic plasticity. CFL1 localises to various cellular components including the cytoplasm, actin cytoskeleton, and cell projections like lamellipodia and filopodia. Its primary functions include actin filament depolymerisation and severing, thereby facilitating dynamic changes in cell shape and motility. Additionally, CFL1 participates in cellular responses to various stimuli, including growth factors and cytokines, influencing cell polarity and migration.
Importance
CFL1 is relevant to: - The study of synaptic plasticity and memory formation due to its role in dendritic spine dynamics. - Cancer research, as it is implicated in cell motility and metastasis through its regulation of the actin cytoskeleton. - Neurodegenerative diseases, where dysregulation of actin dynamics can affect neuronal health and function. - Developmental biology, as it contributes to processes such as cell polarity and morphogenesis during embryonic development.
Top Products
For researchers investigating CFL1, we recommend two primary antibodies that stand out for their performance and reliability. The first is the well-cited polyclonal antibody, Anti-Cofilin antibody - Loading Control (ab42824), which has garnered 92 citations, reflecting its strong reputation in the field. This antibody is versatile, suitable for Western blotting (WB), immunocytochemistry (ICC), and immunohistochemistry (IHC), making it an excellent choice for various experimental needs.In addition, we offer the recombinant monoclonal antibody, Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979). This product has been validated for use in WB and ICC, providing researchers with the batch-to-batch consistency that recombinant antibodies are known for. With 12 citations, it is also gaining traction among scientists studying CFL1. Together, these antibodies provide robust options for effective CFL1 detection in your research. The Anti-Cofilin antibody ELISA Kit (ab11062), supported by 32 citations, is an excellent option for researchers looking to accurately measure CFL1 levels in their samples.
Abcam Product Citation Summary
The data indicates that CFL1 is frequently studied in the context of various cellular processes, particularly in human and rat models. The applications primarily involve Western blotting, highlighting its role in studies related to cell motility, fibrosis prevention, and cytoskeletal dynamics. The consistent use of Abcam antibodies across different studies suggests their reliability in detecting CFL1 in various experimental setups.
Abcam Product Citation Table
Function
Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed:21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).
Post-translational modifications
Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells (By similarity). Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal (By similarity). Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2.
Sequence Similarities
Belongs to the actin-binding proteins ADF family.
Tissue Specificity
Widely distributed in various tissues.
Cellular localization
- Nucleus matrix
- Cytoplasm
- Cytoskeleton
- Cell projection
- Ruffle membrane
- Peripheral membrane protein
- Cytoplasmic side
- Cell projection
- Lamellipodium membrane
- Peripheral membrane protein
- Cytoplasmic side
- Cell projection
- Lamellipodium
- Cell projection
- Growth cone
- Cell projection
- Axon
- Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
Alternative names
CFL, CFL1, Cofilin-1, 18 kDa phosphoprotein, p18