CHAF1B
Developmental stage
Active complex is found in G1, S and G2 phases.
Function
Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.
Post-translational modifications
Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
Sequence Similarities
Belongs to the WD repeat HIR1 family.
Cellular localization
- Nucleus
- Cytoplasm
- DNA replication foci. Cytoplasmic in M phase.
Alternative names
CAF1A, CAF1P60, MPHOSPH7, MPP7, CHAF1B, Chromatin assembly factor 1 subunit B, CAF-1 subunit B, Chromatin assembly factor I p60 subunit, M-phase phosphoprotein 7, CAF-I 60 kDa subunit, CAF-I p60