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CHEK2

Function

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition (PubMed:25361978).

Involvement in disease

Li-Fraumeni syndrome 2

LFS2

A highly penetrant familial cancer syndrome that in its classic form is defined by the existence of a proband affected by a sarcoma before 45 years with a first degree relative affected by any tumor before 45 years and another first degree relative with any tumor before 45 years or a sarcoma at any age. Other clinical definitions for LFS have been proposed (PubMed:8118819 and PubMed:8718514) and called Li-Fraumeni like syndrome (LFL). In these families affected relatives develop a diverse set of malignancies at unusually early ages. Four types of cancers account for 80% of tumors occurring in TP53 germline mutation carriers: breast cancers, soft tissue and bone sarcomas, brain tumors (astrocytomas) and adrenocortical carcinomas. Less frequent tumors include choroid plexus carcinoma or papilloma before the age of 15, rhabdomyosarcoma before the age of 5, leukemia, Wilms tumor, malignant phyllodes tumor, colorectal and gastric cancers.

None

The disease is caused by variants affecting the gene represented in this entry.

Prostate cancer

PC

A malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.

None

Disease susceptibility is associated with variants affecting the gene represented in this entry.

Osteogenic sarcoma

OSRC

A sarcoma originating in bone-forming cells, affecting the ends of long bones.

None

The gene represented in this entry may be involved in disease pathogenesis.

Breast cancer

BC

A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.

None

Disease susceptibility is associated with variants affecting the gene represented in this entry.

Post-translational modifications

Phosphorylated. Phosphorylated at Ser-73 by PLK3 in response to DNA damage, promoting phosphorylation at Thr-68 by ATM and the G2/M transition checkpoint. Phosphorylation at Thr-68 induces homodimerization. Autophosphorylates at Thr-383 and Thr-387 in the T-loop/activation segment upon dimerization to become fully active and phosphorylate its substrates like for instance CDC25C. DNA damage-induced autophosphorylation at Ser-379 induces CUL1-mediated ubiquitination and regulates the pro-apoptotic function. Phosphorylation at Ser-456 also regulates ubiquitination. Phosphorylated by PLK4.

Ubiquitinated. CUL1-mediated ubiquitination regulates the pro-apoptotic function. Ubiquitination may also regulate protein stability. Ubiquitinated by RNF8 via 'Lys-48'-linked ubiquitination.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.

Tissue specificity

High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.

Cellular localization

  • Isoform 2
  • Nucleus
  • Isoform 10 is present throughout the cell.
  • Isoform 4
  • Nucleus
  • Isoform 7
  • Nucleus
  • Isoform 9
  • Nucleus
  • Isoform 12
  • Nucleus
  • Nucleus
  • PML body
  • Nucleus
  • Nucleoplasm
  • Recruited into PML bodies together with TP53.

Alternative names

  • Serine/threonine-protein kinase Chk2
  • CHK2 checkpoint homolog
  • Cds1 homolog
  • Checkpoint kinase 2
  • Hucds1
  • hCds1
  • CHEK2
  • CHK2
  • RAD53
  • CDS1

Target type

Proteins

Primary research area

Oncology

Other research areas

  • Epigenetics

Molecular weight

60915Da

We found 19 products in 4 categories

Primary Antibodies

Target

Reactive species

Assay Kits

Target

Reactive species

Proteins & Peptides

Target

Species of origin

Nature

Cell Lines & Lysates

Target

Cell type

Species or organism

Search our catalogue for 'CHEK2' (19)

Products

ab109413

Anti-Chk2 antibody [EPR4325]

20ul selling size
RabMAb
Recombinant
KO Validated

ab207446

Anti-Chk2 antibody [EPR19482]

20ul selling size
RabMAb
Recombinant
KO Validated

ab133505

Anti-Chk2 antibody [EPR5528]

20ul selling size
RabMAb
Recombinant
KO Validated

ab227998

Anti-Chk2 antibody [EPR4325] - BSA and Azide free

RabMAb
Recombinant
KO Validated

ab251477

Anti-Chk2 antibody [EPR19482] - BSA and Azide free

RabMAb
Recombinant
KO Validated

ab200762

Alexa Fluor® 488 Anti-Chk2 antibody [EPR4325]

RabMAb
Recombinant
KO Validated

ab248527

Anti-Chk2 antibody [EPR5528] - BSA and Azide free

RabMAb
Recombinant
KO Validated