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CNR2

GeneName

CNR2

Summary

CNR2, also known as CB2 or cannabinoid receptor 2, is a 40kDa G protein-coupled receptor primarily expressed in the immune system and the central nervous system, particularly in the brain regions associated with pain and inflammation. It is localised in various cellular components, including the plasma membrane, dendrites, and the endoplasmic reticulum. CNR2 plays a vital role in mediating the effects of cannabinoids, influencing processes such as immune response, inflammatory response, and synaptic transmission. Its activation is involved in the regulation of leukocyte chemotaxis and the modulation of synaptic signalling through endocannabinoids.

Importance

CNR2 is relevant to: - The modulation of immune responses, making it a target for therapies in autoimmune diseases and inflammation. - Pain management, as it is implicated in pain signalling pathways and may offer alternatives to traditional analgesics. - Neurological research, particularly in conditions like multiple sclerosis, where cannabinoid signalling may influence disease progression. - The study of addiction and substance use disorders, given its role in the response to amphetamines and other substances.

Top Products

For researchers investigating CNR2, we highly recommend the well-cited polyclonal antibody, Anti-Cannabinoid Receptor II antibody (ab3561). This antibody has garnered 93 citations, reflecting its reliability and trust within the scientific community. It is particularly effective for Western blotting (WB), immunohistochemistry (IHC), and immunocytochemistry (ICC), making it a versatile tool for various experimental needs. Its proven performance in these applications makes it an excellent choice for those studying the nuances of CNR2.

Abcam Product Citation Summary

The CNR2 target has been studied in various human and mouse tissues, including bone marrow mesenchymal stem cells, lung tissue, and heart tissue. The applications of the Abcam antibody ab3561 include Western blotting and immunohistochemistry, indicating its versatility in detecting CNR2 in different experimental contexts.

Abcam Product Citation Table

Product Code
Species
Application
Study Context
PMID
ab3561
Human
WB, ICC-IF
Bone marrow mesenchymal stem cells
25685815
ab3561
Mouse
WB
Lung tissue
29262578
ab3561
Human
IHC
Heart tissue from women under and over 50 years old
30286717

Function

Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.

Post-translational modifications

Constitutively phosphorylated on Ser-352; phosphorylation increases cell internalization and desensitizes the receptor.

Sequence Similarities

Belongs to the G-protein coupled receptor 1 family.

Tissue Specificity

Preferentially expressed in cells of the immune system with higher expression in B-cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spleen, peripheral blood mononuclear cells, and thymus. PubMed:14657172 could not detect expression in normal brain. Expressed in brain by perivascular microglial cells and dorsal root ganglion sensory neurons (at protein level). Two isoforms are produced by alternative promoter usage and differ only in the 5' UTR: isoform CB2A is observed predominantly in testis with some expression in brain, while isoform CB2B is predominant in spleen and leukocytes.

Cellular localization

Alternative names

CB2A, CB2B, CNR2, Cannabinoid receptor 2, CB-2, CB2, hCB2, CX5

swissprot:P34972 omim:605051 entrezGene:1269