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COL3A1

GeneName

COL3A1

Summary

COL3A1, also known as collagen III, is a 139kDa protein that forms a crucial component of the extracellular matrix (ECM). It is primarily expressed in connective tissues and is secreted into the extracellular space, where it contributes to the structural integrity and tensile strength of tissues. COL3A1 is involved in various biological processes, including collagen fibril organisation, cell-matrix adhesion, and the morphogenesis of smooth muscle tissue and the aorta. Additionally, it plays roles in wound healing, fibroblast proliferation, and the development of various organs, such as the heart and lungs. The protein interacts with integrins and other growth factors, influencing cellular responses and tissue homeostasis.

Importance

COL3A1 is relevant to: - Understanding connective tissue disorders, such as Ehlers-Danlos syndrome, due to its role in collagen structure and function - Investigating cardiovascular diseases, given its involvement in aorta development and vascular integrity - Exploring mechanisms of wound healing and tissue regeneration, as it is crucial for ECM organisation - Studying developmental biology, particularly in relation to organogenesis and embryonic development - Researching its role in cancer biology, as alterations in collagen composition can affect tumour microenvironments and metastasis

Top Products

For researchers investigating COL3A1, we recommend two excellent primary antibodies that cater to a variety of applications. The first is the highly regarded polyclonal antibody, Anti-Collagen I + Collagen III antibody (ab34710), which has garnered an impressive 2238 citations, underscoring its reliability in Western blotting (WB) and immunohistochemistry (IHC). This antibody is a trusted choice for those studying collagen types I and III.In addition, we offer the recombinant monoclonal antibody, Anti-Collagen III antibody [EPR17673] (ab184993). This product has been validated for use in multiple applications, including WB, immunocytochemistry (ICC), flow cytometry (FC), and immunoprecipitation (IP). With 147 citations, it is gaining traction in the research community and provides the batch-to-batch consistency that is often essential for experimental reproducibility. Together, these antibodies provide robust options for COL3A1 research. The Native Human Collagen III protein ELISA Kit (ab7535), supported by 6 citations, is an excellent option for researchers looking to accurately measure COL3A1 levels in their samples.

Abcam Product Citation Summary

The data indicates a significant focus on the role of COL3A1 in various fibrotic conditions and tissue responses across multiple species, particularly in Rattus norvegicus and Mus musculus. Studies often involve Western blotting and immunohistochemistry applications, highlighting the importance of COL3A1 in renal, cardiac, and pulmonary fibrosis, as well as its expression in response to various stimuli such as Ghrelin and TGF-β1. The research underscores COL3A1's relevance in understanding collagen dynamics in health and disease.

Abcam Product Citation Table

ab184993
Rat
WB
Renal interstitial ECM accumulation
32714415
ab184993
Human
WB
Gene expression
30704535
ab6310
Human
IF
Effect of adiponectin on type III collagen expression
27428951
ab6310
Rat
ICC-IF
Collagen deposition and aortic fibrosis
32699285
ab7778
Mouse
IHC
Hepatic fibrosis post Schistosoma japonicum infection
29321784
ab7778
Human
IHC
Decellularization
28717194
ab7778
Rat
IHC
Atherosclerotic right carotid arteries
28765576
ab7778
Human
WB
Collagen expression over time
29085840
ab7778
Rat
WB
Glucose exposure effects
28182710
ab7778
Mouse
WB
Renal autophagy
28182710
ab7778
Human
WB
Macrophage profibrogenesis
26436920
ab7778
Mouse
WB
Cardiac fibrosis
30430766
ab7778
Mouse
WB
Spontaneous lung fibrosis
31358769
ab7778
Human
WB
Collagen production in preeclampsia
30804321
ab7778
Rat
WB
Myocardial fibrosis
35592403
ab7778
Rat
WB
Heart failure
39061056
ab7778
Human
WB
Collagen distribution in cardiac tissue
27747234
ab7778
Mouse
WB
Collagen production in CFTR deficiency
31998800
ab7778
Human
WB
Effect of cell sex on ECM protein expression
28676082
ab7778
Mouse
WB
ECM deposition
31929748
ab7778
Mouse
WB
Renal fibroblast activation
31929748
ab7778
Rat
IHC
Tendon analysis
31717431
ab7778
Rat
IHC
Tendon healing
31717431
ab7778
Human
WB
Collagen expression regulation by leptin
29436483
ab7778
Human
WB
Collagen expression in scars
31413962
ab7778
Human
WB
Collagen expression in hypertrophic cardiomyopathy
30699940
ab7778
Human
WB
Collagen expression in cardiac fibroblasts
31438862
ab7778
Human
WB
Collagen expression in smooth muscle progenitor cells
28676082
ab7778
Human
WB
Collagen expression in BMSC
29038440
ab7778
Human
WB
Collagen expression in conditioned medium
29038440
ab7778
Mouse
WB
Collagen expression in lung tissues
32448163
ab7778
Mouse
WB
Collagen expression in kidney tissue
31929748
ab7778
Mouse
WB
Collagen expression in heart tissue
31680453
ab7778
Mouse
WB
Collagen expression in lung sections
31358769
ab7778
Rat
WB
Collagen expression in kidney samples
30713574
ab7778
Rat
WB
Collagen expression in kidney tissue
30713574

Domain

The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.

Function

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.

Involvement in disease

Ehlers-Danlos syndrome, vascular type

EDSVASC

A severe form of Ehlers-Danlos syndrome, a group of connective tissue disorders characterized by skin hyperextensibility, articular hypermobility, and tissue fragility. EDSVASC is an autosomal dominant disease characterized by joint and dermal manifestations as in other forms of the syndrome, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.

None

The disease is caused by variants affecting the gene represented in this entry.

Polymicrogyria with or without vascular-type Ehlers-Danlos syndrome

PMGEDSV

An autosomal recessive disorder with a highly variable phenotype and onset in early childhood. Disease features include cobblestone-like malformation of the cortex, polymicrogyria, intellectual and motor developmental delay, small joint hypermobility, vascular fragility, aneurysms, thin translucent skin and easy bruising, congenital heart defects, and foot deformities. Early death due to vascular dissection may occur.

None

The disease is caused by variants affecting the gene represented in this entry.

Post-translational modifications

Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.

Sequence Similarities

Belongs to the fibrillar collagen family.

Cellular localization

Alternative names

Collagen alpha-1(III) chain, COL3A1

swissprot:P02461 omim:120180 entrezGene:1281