colA
Domain
The mature protein has 4 domains; a metalloprotease domain (S1, approximately residues 87-761), S2 (762-860, equivalent to PKD), and 2 collagen-binding domains S3a (865-979) and S3b (992-1104) (PubMed:9922257). The metalloprotease S1 domain is composed of 3 subdomains which together resemble a saddle; an activator domain (residues 93-367), the catalytic peptidase subdomain (377-646) and a helper subdomain (654-767) (By similarity).
Function
Clostridial collagenases are among the most efficient degraders of eukaryotic collagen known; saprophytes use collagen as a carbon source while pathogens additionally digest collagen to aid in host colonization. Has both tripeptidylcarboxypeptidase on Gly-X-Y and endopeptidase activities; the endopeptidase cuts within the triple helix region of collagen while tripeptidylcarboxypeptidase successively digests the exposed ends, thus clostridial collagenases can digest large sections of collagen (By similarity).
Sequence Similarities
Belongs to the peptidase M9B family. Collagenase subfamily.
Cellular localization
- Secreted
Alternative names
CPE0173, colA, Collagenase ColA, 120 kDa collagenase, Microbial collagenase