CREB1
GeneName
CREB1
Summary
CREB1, also known as CREB or CREB-1, is a 35kDa transcription factor that plays a pivotal role in mediating cellular responses to various stimuli, including hormones and growth factors. It is primarily localised in the nucleus, where it binds to cAMP response elements in DNA to regulate gene expression. CREB1 is part of several complexes, including the ATF4-CREB1 transcription factor complex and the RNA polymerase II transcription regulator complex. This protein is involved in multiple biological processes such as axonogenesis, memory, and hormone secretion, and is essential for the transcriptional regulation of genes associated with neuronal plasticity and metabolic functions.
Importance
CREB1 is relevant to: - Neurobiology, particularly in understanding mechanisms of memory and learning due to its role in synaptic plasticity - Endocrinology, as it regulates hormone secretion and cellular responses to growth factors - Cancer research, given its involvement in gene expression regulation that can affect cell proliferation and survival - Metabolic disorders, through its influence on lipid biosynthesis and fat cell differentiation - Developmental biology, as it plays a role in processes like lung saccule development and osteoclast differentiation
Top Products
For researchers investigating CREB1, we highly recommend the top-selling recombinant monoclonal antibody, Anti-CREB antibody [E306] (ab32515). This antibody has been validated in knockout models, ensuring its reliability in various experimental settings. It is particularly effective for Western blotting (WB) and immunohistochemistry (IHC), making it a versatile tool for studying CREB1. With 190 citations, this antibody is well-regarded in the research community, reflecting its trusted performance in the field.
Abcam Product Citation Summary
The data indicates that the CREB1 antibody (ab32515) has been effectively used in various studies involving Western blotting across different contexts, primarily in mouse and rat models. The studies focus on significant biological processes such as the effects of fluoxetine administration, oxygen-glucose deprivation, and the cAMP/PKA/CREB/BDNF pathway, highlighting the relevance of CREB1 in neuronal and osteoblastic functions.
Abcam Product Citation Table
Function
Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters (By similarity). Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation (PubMed:14536081). Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells (By similarity). Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling (By similarity).
Involvement in disease
Angiomatoid fibrous histiocytoma
AFH
A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis.
None
The gene represented in this entry may be involved in disease pathogenesis. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
A CREB1 mutation has been found in a patient with multiple congenital anomalies consisting of agenesis of the corpus callosum, cerebellar hypoplasia, severe neonatal respiratory distress refractory to surfactant, thymus hypoplasia, and thyroid follicular hypoplasia.
Post-translational modifications
Stimulated by phosphorylation. Phosphorylation of both Ser-119 and Ser-128 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-119 allows CREBBP binding. In liver, phosphorylation is induced by fasting or glucagon in a circadian fashion (By similarity). CREBL2 positively regulates phosphorylation at Ser-119 thereby stimulating CREB1 transcriptional activity (By similarity). Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-119. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-128. Phosphorylation of Ser-128 blocks CREB-mediated transcription even when Ser-119 is phosphorylated. Phosphorylated by CaMK1 (By similarity). Phosphorylation of Ser-257 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-119 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli. Phosphorylated by TSSK4 on Ser-119 (PubMed:15964553).
Sumoylated with SUMO1. Sumoylation on Lys-290, but not on Lys-271, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
Sequence Similarities
Belongs to the bZIP family.
Cellular localization
- Nucleus
Alternative names
Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1
Database links
swissprot:P16220 omim:123810 entrezGene:1385
Other research areas
- Epigenetics
- Immuno-oncology
- Immunology & Infectious Disease
- Oncology