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Developmental stage

Not detected at 13.5 dpc. Detected in mitotically arrested gonocytes of 14.5 dpc embryos. From 17.5 dpc to newborn, it is expressed in some centrally located gonocytes and cells present at the periphery of the developing seminiferous tubules. Present in nuclei of spermatogonia from 15 days after birth to adulthood.

Function

Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites. Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.

Sequence similarities

Belongs to the CTCF zinc-finger protein family.

Tissue specificity

Testis-specific.

Cellular localization

  • Cytoplasm
  • Nucleus

Alternative names

Boris, Transcriptional repressor CTCFL, Brother of the regulator of imprinted sites, CCCTC-binding factor, CTCF paralog, CTCF-like protein

Target type

Proteins

Primary research area

Epigenetics

Molecular weight

73108Da

We found 1 product in 1 category

Primary Antibodies

Target

Application

Reactive species

Search our catalogue for 'BORIS' (1)

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