CTSB
GeneName
CTSB
Summary
CTSB, also known as cathepsin B, APPS, or cathepsin B1, is a 38kDa cysteine protease that is primarily expressed in lysosomes and is involved in various cellular processes. It is localised to multiple cellular compartments including the endolysosome lumen, extracellular space, and apical plasma membrane. CTSB plays a critical role in proteolysis, facilitating the degradation of extracellular matrix components such as collagen and proteoglycans. Additionally, it is implicated in the regulation of apoptotic processes and has functions in epithelial cell differentiation and responses to thyroid hormones.
Importance
CTSB is relevant to: - Cancer biology due to its role in tumour progression and metastasis through extracellular matrix remodelling - Neurodegenerative diseases as it may contribute to the processing of amyloid precursor proteins - Inflammatory responses where its proteolytic activity influences immune cell function and tissue remodelling - The study of host-pathogen interactions, given its involvement in symbiont entry into host cells
Top Products
For researchers investigating CTSB, we recommend two excellent primary antibodies. The first is the well-cited monoclonal antibody, Anti-Cathepsin B antibody [CA10] (ab58802), which has garnered 105 citations, highlighting its reliability in Western blotting (WB) and flow cytometry (FC). Additionally, we offer the recombinant antibody, Anti-Cathepsin B antibody [EPR4323] (ab125067), which is validated for use in Western blotting (WB), immunohistochemistry (IHC), and immunocytochemistry (ICC). With 27 citations, this recombinant product ensures batch-to-batch consistency, making it a robust choice for your CTSB research needs. The Recombinant Human Cathepsin B protein (Active) ELISA Kit (ab283434) is a reliable option for researchers looking to measure Cathepsin B in their experiments, supported by 1 citation.
Abcam Product Citation Summary
The data indicates that CTSB is being studied across various species, including sheep, mice, and goats, with a focus on different biological contexts such as obesity, exercise training, and DNA damage. The predominant application for detecting CTSB is Western Blotting, highlighting its importance in protein analysis in these studies.
Abcam Product Citation Table
Function
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (PubMed:12220505). Cleaves matrix extracellular phosphoglycoprotein MEPE (PubMed:12220505). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity). Has also been implicated in tumor invasion and metastasis (PubMed:3972105).
Involvement in disease
Keratolytic winter erythema
KWE
An autosomal dominant genodermatosis characterized by recurrent episodes of palmoplantar erythema and epidermal peeling presenting seasonal variation. KWE manifests during childhood. Skin lesions may spread to the dorsum of hands and feet and to the interdigital spaces. Lower legs, knees and thighs may also be involved. A less common finding is a slowly migratory, annular erythema that is seen mostly on the extremities. Between flares, the skin can appear unremarkable. Itching can occur, and hyperhidrosis, associated with a pungent odor, is invariably present. Formation of vesicles is rare, whereas keratolysis that causes the formation of dry blisters is regularly seen. Cold weather, moisture, febrile diseases, and physical and mental stress can trigger exacerbations. In severely affected individuals, skin manifestations persist unremittingly. Penetrance of the disease is high, but expressivity is variable, even within the same family.
None
The gene represented in this entry is involved in disease pathogenesis. Tandem duplications in a non-coding genomic region containing an active enhancer element for CTSB result in CTSB abnormal expression with pathological consequences.
Sequence Similarities
Belongs to the peptidase C1 family.
Tissue Specificity
Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum.
Cellular localization
- Lysosome
- Melanosome
- Secreted
- Extracellular space
- Apical cell membrane
- Peripheral membrane protein
- Extracellular side
- Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Localizes to the lumen of thyroid follicles and to the apical membrane of thyroid epithelial cells (By similarity).
Alternative names
CPSB, CTSB, Cathepsin B, APP secretase, Cathepsin B1, APPS