CYP2E1
GeneName
CYP2E1
Summary
CYP2E1, also known as CYP2E or cytochrome P450 2E1, is a 57 kDa enzyme that is primarily located in the endoplasmic reticulum membrane and mitochondria of liver cells, but is also found in other tissues. It plays a crucial role in the metabolism of various xenobiotics and endogenous compounds, including the oxidation of small molecules such as alcohols and hydrocarbons. CYP2E1 is involved in several metabolic pathways, including the epoxygenase P450 pathway and the metabolism of halogenated hydrocarbons. The enzyme exhibits monooxygenase and oxidoreductase activities, facilitating the incorporation of oxygen into substrates, which is essential for detoxification processes.
Importance
CYP2E1 is relevant to: - The metabolism of drugs and environmental toxins, influencing pharmacokinetics and toxicity profiles. - Alcohol metabolism and its role in alcohol-related liver diseases, as it can generate reactive oxygen species. - The biotransformation of various carcinogens, contributing to cancer risk assessment. - Disorders related to lipid metabolism, given its involvement in long-chain fatty acid biosynthesis and hydroxylation.
Top Products
For researchers investigating CYP2E1, we recommend two primary antibodies that offer excellent performance in various applications. The first is the well-cited polyclonal antibody, Anti-Cytochrome P450 2E1 antibody (ab53945), which has garnered 4 citations and is particularly effective in Western blotting (WB) and immunohistochemistry (IHC). This antibody is a trusted choice for those looking to study CYP2E1 in detail. Additionally, we offer the recombinant antibody, Anti-Cytochrome P450 2E1 antibody [EPR28698-543] (ab324160), which also excels in WB and IHC. As a recombinant product, it ensures batch-to-batch consistency, making it an ideal option for researchers who require reliable results.
Abcam Product Citation Summary
The data indicates that CYP2E1 is being studied in the context of human LX2 cells, particularly in response to exposure to isoniazid (INH). This suggests a focus on the role of CYP2E1 in drug metabolism or toxicity.
Abcam Product Citation Table
Function
A cytochrome P450 monooxygenase involved in the metabolism of fatty acids (PubMed:10553002, PubMed:18577768). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:10553002, PubMed:18577768). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids (PubMed:10553002, PubMed:18577768). May be involved in the oxidative metabolism of xenobiotics (Probable).
Pathway
Lipid metabolism; fatty acid metabolism.
Sequence Similarities
Belongs to the cytochrome P450 family.
Cellular localization
- Endoplasmic reticulum membrane
- Peripheral membrane protein
- Microsome membrane
- Peripheral membrane protein
- Mitochondrion inner membrane
- Peripheral membrane protein
- Post-translationally targeted to mitochondria. TOMM70 is required for the translocation across the mitochondrial outer membrane. After translocation into the matrix, associates with the inner membrane as a membrane extrinsic protein.
Alternative names
CYP2E, CYP2E1, Cytochrome P450 2E1, 4-nitrophenol 2-hydroxylase, CYPIIE1, Cytochrome P450-J