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Cystine/glutamate transporter

GeneName

SLC7A11

Summary

SLC7A11, also known as xCT or solute carrier family 7 member 11, is a 55 kDa transmembrane protein primarily localised to the plasma membrane and various cellular projections, including astrocyte projections and microvillus membranes. It functions as a cystine-glutamate antiporter, facilitating the transport of cystine into cells while exporting glutamate. This protein plays a crucial role in maintaining intracellular glutamate homeostasis and is involved in the glutathione metabolic process, which is essential for cellular defence against oxidative stress. SLC7A11 is expressed in various tissues and is particularly relevant in the brain, where it contributes to synaptic transmission and neuronal health.

Importance

SLC7A11 is relevant to: - The regulation of oxidative stress responses, impacting neurodegenerative diseases and cellular survival - Ferroptosis, a form of regulated cell death linked to various pathologies, including cancer - The modulation of glutamate levels, which is critical for neurotransmission and implicated in psychiatric disorders - The transport of amino acids, influencing metabolic processes and cellular signalling - Developmental processes in the lung and striatum, highlighting its role in tissue development and homeostasis

Top Products

For researchers investigating SLC7A11, we recommend two excellent primary antibodies. The first is the well-cited polyclonal antibody, Anti-xCT antibody (ab37185), which has garnered 147 citations, reflecting its reliability in immunohistochemistry (IHC) and immunocytochemistry (ICC). Additionally, we offer the recombinant antibody, Anti-xCT antibody [EPR27115-64] (ab307601), which has been validated in knockout models and is suitable for a broader range of applications, including western blotting (WB), IHC, ICC, immunoprecipitation (IP), and flow cytometry (FC). With 86 citations, this recombinant antibody is an excellent choice for researchers seeking consistency and versatility in their experiments.

Abcam Product Citation Summary

The data indicates that SLC7A11 is being investigated in various contexts, including cancer sensitivity, mitochondrial function, and neuroinflammation. The use of Abcam antibody ab37185 in multiple species, particularly humans and mice, highlights its relevance in studies related to transporter expression and ferroptosis.

Abcam Product Citation Table

Product Code
Species
Application
Study Context
PMID
ab37185
Human
WB
Sensitivity to vorinostat
32235498
ab37185
Human
WB
Mitochondrial function and viability
28429737
ab37185
Mouse
WB, ICC-IF
Transporter expression
25709567
ab37185
Mouse
WB, ICC-IF
Cystine/cysteine and glutamate transporters expression
25709567
ab37185
Mouse
WB, ICC-IF
Cystine, cysteine, and glutamate transporters in the male hippocampus
25709567
ab37185
Human
WB
LPS activation in U-937 monocytes
21639880
ab37185
Rat
WB, IHC
Experimental autoimmune encephalomyelitis
21639880
ab37185
Human
WB
Ferroptosis
32685019

Function

Heterodimer with SLC3A2, that functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane (PubMed:11133847, PubMed:11417227, PubMed:14722095, PubMed:15151999, PubMed:34880232, PubMed:35245456, PubMed:35352032). Provides L-cystine for the maintenance of the redox balance between extracellular L-cystine and L-cysteine and for the maintenance of the intracellular levels of glutathione that is essential for cells protection from oxidative stress (By similarity). The transport is sodium-independent, electroneutral with a stoichiometry of 1:1, and is drove by the high intracellular concentration of L-glutamate and the intracellular reduction of L-cystine (PubMed:11133847, PubMed:11417227). In addition, mediates the import of L-kynurenine leading to anti-ferroptotic signaling propagation required to maintain L-cystine and glutathione homeostasis (PubMed:35245456). Moreover, mediates N-acetyl-L-cysteine uptake into the placenta leading to subsequently down-regulation of pathways associated with oxidative stress, inflammation and apoptosis (PubMed:34120018). In vitro can also transport L-aspartate (PubMed:11417227). May participate in astrocyte and meningeal cell proliferation during development and can provide neuroprotection by promoting glutathione synthesis and delivery from non-neuronal cells such as astrocytes and meningeal cells to immature neurons (By similarity). Controls the production of pheomelanin pigment directly (By similarity).

Post-translational modifications

Ubiquitinated by TRIM26; leading to proteasomal degradation.

Sequence Similarities

Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.

Tissue Specificity

Expressed in term placenta and primary term cytotrophoblast (PubMed:34120018). Expressed mainly in the brain, but also in pancreas (PubMed:11417227).

Cellular localization

Alternative names

Cystine/glutamate transporter, Amino acid transport system xc-, Calcium channel blocker resistance protein CCBR1, Solute carrier family 7 member 11, xCT, SLC7A11

swissprot:Q9UPY5 entrezGene:23657 omim:607933