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Function

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.

Post-translational modifications

The heme prosthetic group could be coordinated with residues of the light chain, the heavy chain, or both, and it is possible that more than one heme is present per cytochrome b-245.

Phosphorylation at Thr-147 enhances NADPH oxidase activity by promoting p47phox binding.

Ubiquitinated at Lys-149 likely by RNF145.

Sequence similarities

Belongs to the p22phox family.

Tissue specificity

The strongest level of expression is found in kidney, peritoneal neutrophils and peritoneal macrophages, and a lower level in spleen and small intestine. Very low level of expression can be noted in brain, liver, testis, and heart.

Cellular localization

  • Cell membrane
  • As unassembled monomer may localize to the endoplasmic reticulum.

Alternative names

Cytochrome b-245 light chain, Cytochrome b(558) alpha chain, Cytochrome b558 subunit alpha, Neutrophil cytochrome b 22 kDa polypeptide, Superoxide-generating NADPH oxidase light chain subunit, p22 phagocyte B-cytochrome, p22-phox, p22phox, Cyba

Target type

Proteins

Primary research area

Neuroscience

Molecular weight

20748Da

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