DBP
GeneName
DBP
Summary
DBP, also known as DNA binding protein, ICP8, or ORF6, is a 34 kDa protein that is primarily expressed in the nucleus and is associated with chromatin and the RNA polymerase II transcription regulator complex. It functions as a transcription factor, specifically binding to DNA sequences to regulate transcription by RNA polymerase II. DBP plays a role in the positive regulation of transcription, particularly involved in liver development and rhythmic processes, indicating its importance in the temporal regulation of gene expression.
Importance
DBP is relevant to: - Understanding liver development and function through its role in transcription regulation - Investigating circadian rhythms and their impact on gene expression - Exploring mechanisms of RNA polymerase II-mediated transcription, which is fundamental to many cellular processes - Studying viral proteins, as DBP is known to interact with viral DNA in some contexts, providing insights into viral replication and pathogenesis
Top Products
For researchers investigating DBP, we recommend two primary antibodies that cater to different experimental needs. The first is the well-cited monoclonal antibody, Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (ab20194), which has garnered 52 citations and is particularly effective for immunocytochemistry (ICC). This antibody is a trusted choice for those focusing on DBP in cellular contexts. Additionally, we offer the recombinant antibody, Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112). This product is validated for a broader range of applications, including Western blotting (WB), ICC, flow cytometry (FC), and immunoprecipitation (IP). The recombinant nature of this antibody ensures batch-to-batch consistency, making it an excellent option for researchers requiring reliable and versatile detection of DBP.
Abcam Product Citation Summary
The data indicates that the Abcam antibody ab20194 has been consistently used to detect DBP in Chlorocebus sabaeus (Green monkey) through Western blotting and immunocytochemistry-immunofluorescence (ICC-IF). This suggests a focus on understanding the role of DBP in this species, potentially in various biological contexts.
Abcam Product Citation Table
Function
This transcriptional activator recognizes and binds to the sequence 5'-RTTAYGTAAY-3' found in the promoter of genes such as albumin, CYP2A4 and CYP2A5. It is not essential for circadian rhythm generation, but modulates important clock output genes. May be a direct target for regulation by the circadian pacemaker component clock. May affect circadian period and sleep regulation.
Sequence Similarities
Belongs to the bZIP family. PAR subfamily.
Tissue Specificity
Ubiquitously expressed. Expressed in the suprachiasmatic nuclei (SCN) and in most peripheral tissues, with a strong circadian rhythmicity.
Cellular localization
- Nucleus
Alternative names
D site-binding protein, Albumin D box-binding protein, Albumin D-element-binding protein, Tax-responsive enhancer element-binding protein 302, TaxREB302, DBP