DDX42
Function
ATP-dependent RNA helicase that binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures (PubMed:16397294). Unwinding is promoted in the presence of single-strand binding proteins (PubMed:16397294). Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein (PubMed:16397294). ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands (PubMed:16397294). Required for assembly of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs: DDX42 associates transiently with the SF3B subcomplex of the 17S U2 SnRNP complex and is released after fulfilling its role in the assembly of 17S U2 SnRNP (PubMed:12234937, PubMed:36797247). Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2 (PubMed:19377511). Relocalizes TP53BP2 to the cytoplasm (PubMed:19377511).
Sequence Similarities
Belongs to the DEAD box helicase family. DDX42 subfamily.
Tissue Specificity
Expressed in several cell lines (at protein level). Expressed in liver, lung, tonsil, thymus, muscle and pancreatic islets.
Cellular localization
- Cytoplasm
- Nucleus
- Isoform 2
- Nucleus
- Cajal body
- Nucleus speckle
Alternative names
ATP-dependent RNA helicase DDX42, DEAD box protein 42, RNA helicase-like protein, RNA helicase-related protein, SF3b DEAD box protein, Splicing factor 3B-associated 125 kDa protein, RHELP, RNAHP, SF3b125, DDX42