DGKZ
Domain
The PDZ-binding motif mediates interaction with PDZ domain-containing proteins like SNTG1 and SNX27.
Function
Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:15544348, PubMed:18004883, PubMed:19744926, PubMed:22108654, PubMed:22627129, PubMed:23949095, PubMed:9159104). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:15544348, PubMed:18004883, PubMed:19744926, PubMed:22108654, PubMed:22627129, PubMed:23949095, PubMed:9159104). Also plays an important role in the biosynthesis of complex lipids (Probable). Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species (PubMed:19744926, PubMed:22108654, PubMed:9159104). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group (PubMed:15544348, PubMed:19744926, PubMed:22627129). The biological processes it is involved in include T cell activation since it negatively regulates T-cell receptor signaling which is in part mediated by diacylglycerol (By similarity). By generating phosphatidic acid, stimulates PIP5KIA activity which regulates actin polymerization (PubMed:15157668). Through the same mechanism could also positively regulate insulin-induced translocation of SLC2A4 to the cell membrane (By similarity).
Isoform 1
Regulates RASGRP1 activity.
Isoform 2
Does not regulate RASGRP1 activity.
Pathway
Lipid metabolism; glycerolipid metabolism.
Post-translational modifications
Phosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta.
Sequence Similarities
Belongs to the eukaryotic diacylglycerol kinase family.
Tissue Specificity
Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas.
Isoform 2
Predominantly expressed in muscle.
Cellular localization
- Nucleus
- Cytoplasm
- Cytosol
- Cell membrane
- Cell projection
- Lamellipodium
Alternative names
DAGK6, DGKZ, Diacylglycerol kinase zeta, DAG kinase zeta, Diglyceride kinase zeta, DGK-zeta