DLG1_HUMAN
Domain
The alternatively spliced domain I3 corresponding to amino acids (636-669) of isoform 4 is an EPB41 binding site mediating association to membranes in polarized and non-polarized cells.
The PDZ domains may also mediate association to membranes by binding to EPB41 and ADGRA2 together with the L27 domain that binds CASK and DLG2.
The L27 domain may regulate DLG1 self-association. The N-terminal alternatively spliced region is capable of binding several SH3 domains and also moderates the level of protein oligomerization.
Function
Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. Promotes epithelial cell layer barrier function via maintaining cell-cell adhesion (By similarity). May also play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel. During long-term depression in hippocampal neurons, it recruits ADAM10 to the plasma membrane (PubMed:23676497).
Post-translational modifications
Phosphorylated by MAPK12 (PubMed:20605917). Phosphorylation of Ser-232 regulates association with GRIN2A (By similarity).
Ubiquitinated; by MARCHF2 which results in its degradation.
Sequence Similarities
Belongs to the MAGUK family.
Tissue Specificity
Abundantly expressed in atrial myocardium (at protein level). Expressed in lung fibroblasts, cervical epithelial and B-cells (at protein level). Expressed in the brain (at protein level) (PubMed:23676497). Widely expressed, with isoforms displaying different expression profiles.
Cellular localization
- Cell membrane
- Peripheral membrane protein
- Basolateral cell membrane
- Endoplasmic reticulum membrane
- Postsynaptic density
- Synapse
- Cell membrane
- Sarcolemma
- Apical cell membrane
- Cell junction
- Cytoplasm
- Colocalizes with EPB41 at regions of intercellular contacts. Basolateral in epithelial cells (PubMed:12807908). May also associate with endoplasmic reticulum membranes. Mainly found in neurons soma, moderately found at postsynaptic densities (By similarity).
Alternative names
Disks large homolog 1, Synapse-associated protein 97, hDlg, SAP-97, SAP97, DLG1