DUS2
Domain
Efficient dihydrouridine synthesis requires the presence of both the catalytic domain and the C-terminal RNA-binding DRBM domain.
Function
Catalyzes the NADPH-dependent synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs (PubMed:15994936, PubMed:26429968, PubMed:30149704, PubMed:34798057, PubMed:38680565). Specifically modifies U20 in cytoplasmic tRNAs (PubMed:38680565). Activity depends on the presence of guanosine at position 19 in the tRNA substrate (PubMed:38680565). Negatively regulates the activation of EIF2AK2/PKR (PubMed:18096616).
Sequence Similarities
Belongs to the Dus family. Dus2 subfamily.
Tissue Specificity
Weak expression in heart, placenta and skeletal muscle. Up-regulated in most lung cancer cells (at protein level).
Cellular localization
- Cytoplasm
- Endoplasmic reticulum
- Mainly at the endoplasmic reticulum.
Alternative names
DUS2L, DUS2, tRNA-dihydrouridine(20) synthase [NAD(P)+]-like, Dihydrouridine synthase 2, Up-regulated in lung cancer protein 8, tRNA-dihydrouridine synthase 2-like, URLC8, hDUS2